摘要
目的:探讨建立寒凝证动物模型的最佳温度条件。方法:将50只雌性SD大鼠随机分为A、B、C、D、E 5组,每组10只,A组为对照组,B、C组为-15℃冷冻组,D、E组为-25℃冷冻组,各组均冷冻5 d,每天冷冻4 h。C、E组同样冷冻后,正常喂养5 d。观察大鼠体征和体重,在冷冻结束后第1天和第5天取血检测血液流变学指标。结果:与A组比较,B、C、D、E组大鼠体征变化评分均明显升高,P<0.05;与A组比较,B组和D组冷冻期内大鼠体重下降,P<0.05,E组冷冻结束后体重增加较少,P<0.05;与A组比较,B组与D组全血还原黏度高切明显升高,P<0.05,红细胞刚性指数有升高趋势,但未见统计学差异。结论:每天-25℃、4 h,连续冷冻5 d,可建立寒凝证模型,结合整体体征评分及血液流变学指标评价,符合中医寒凝证候特点。
Objective：To explore the optimum temperature conditions of animal model with cold coagulation syndrome. Methods： Fifty female SD rats were randomized into A,B,C,D and E groups,with 10 cases in each. A group was control group,B and D groups were -15 ℃ frozen groups,C and E groups were -25 ℃ frozen groups. Each group was put in deep freezer 5 hours every day,continous 5 days. D and E groups were fed 5 days. To observe symptoms,weight and hemorheology of rats at the first and fifth days after frozen days. Results：Compared with A group,ouflooking scores of B,C,D,E groups increased remarkably（P〈0.05）. Compared with A group,weight of B,D groups in frozing period reduced siguificantly（P〈0.05）. Frozen after,weight of E group less increase（P（0.05）. Compared with A group,whole blood reductive viscosity high shear of B and D groups were significantly higher（P（0.05）,the erythrocyte rigidity indexes were increased,but there was no significant differences. Conclusion：cold coagulation syndrome models can be established as follow：-25 ℃,4 h,continuous freezer 5 d. Based on the overall symptoms score and hemorheology evaluation,the model has the characteristics of TCM cold coagulation syndrome.
出处
《山东中医药大学学报》
2013年第3期248-250,共3页
Journal of Shandong University of Traditional Chinese Medicine
基金
国家重点基础研究发展计划(973计划)(编号:2012CB518506)
关键词
寒凝证
血液流变学
寒凝表征评分
模型研究
cold coagulation syndrome
hemorheology
cold syndrome characterization score
model study
