摘要
目的观察参附注射液对大鼠脑缺血再灌注后磷酸果糖激酶(PFK)表达的影响,探讨参附注射液对脑的保护机制。方法清洁级雄性SD大鼠90只,随机分为假手术(Sham)组、脑缺血再灌注(IR)组、参附注射液预处理(SFI组),每组30只。采用线栓法制备大鼠大脑中动脉闭塞(MCAO)后再灌注模型,TTC染色测脑梗死面积,并检测PFK活性及PFKmRNA和蛋白的表达。结果参附注射液能明显减少脑缺血再灌注大鼠脑梗死灶的面积。IR组与Sham组比较,PFK活性明显降低(P<0.05),PFK mRNA及蛋白的表达明显增加(P<0.05);SFI组PFK mRNA及蛋白的表达水平及活性明显高于IR组(P<0.05)。结论参附注射液对脑缺血再灌注大鼠脑组织有保护作用,其机制可能与上调PFK表达及活性有关。
Objective To investigate the effect of Shenfu Injection （SFI） on the expression of phosphofructokinase （PFK） in rats after cerebral ischemia-reperfusion injury and to discuss its protective mechanism on brain. Methods Male SD rats were randomly divided into three groups, Sham, cerebral ischemic reperfusion （IR）, and SFI-pretreated （SFI） groups, with 30 rats in each group. Reversible middle cerebral artery occlusion （MCAO） model was produced by intraluminal suture technique. The infarct area of brain was measured by 2,3,5-triphenyl tetrazolium chloride （TTC） staining method; The PFK activity and the expression ofPFK mRNA and protein were detected, respectively. Results SFI could significantly decrease the infarct areas of brain with MCAO in rats. Compared with those in Sham group, the PFK activity decreased （P 〈 0.05） and the expression of PFK mRNA and protein increased in IR group （P 〈 0.05）. The PFK activity and the expression ofPFK mRNA and protein were significantly increased in SFI group compared with IR group （P 〈 0.05）. Conclusion SFI has the protective effects on the brain injury induced by ischemia. Its mechanism may be correlated to up-regnlating the expression and activity of PFK.
出处
《药物评价研究》
CAS
2013年第2期100-103,共4页
Drug Evaluation Research
基金
四川省科技厅资助项目(2010JY0094)
关键词
参附注射液
脑缺血再灌注
磷酸果糖激酶
Shenfu Injection
cerebral ischemia reperfusion
phosphofructokinase
