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丙烯酰胺对大鼠肝脏P4502E1活力的影响

Effects of the Acrylamide on the Activity of P4502E1 in Rat Liver
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摘要 丙烯酰胺(acrylamide,Acr)具有毒性和潜在的致癌性,食品中丙烯酰胺的存在及食用安全性引起了社会的高度重视。选用鼠龄50d的Sprague-Dawley雄性大鼠80只,随机分成4组进行Acr染毒实验,染毒剂量分别为0、25、50、100mg·kg-1,即对照组和低、中、高3个剂量组。染毒采用腹腔注射(对照组使用生理盐水),隔日染毒,连续12周。在第3、6、9、12周末次染毒4h后处死取样。通过测定肝脏微粒体中细胞色素P4502E1(cytochrome P4502El)的活力来观察Acr对大鼠肝脏P4502E1活力的影响。结果表明,肝微粒体P4502E1活力在25mg·kg-1剂量组随染毒时间增加而增强,染毒第12周的酶活力明显高于染毒第3、6、9周。在50、100mg·kg-1剂量组,P4502E1酶活力在染毒第6周出现峰值。研究P4502E1酶活力在肝脏丙烯酰胺代谢过程中的变化,为进一步探讨丙烯酰胺代谢途径及其毒性作用奠定了基础。 Presence of acrylamide (Acr), which is toxic and potentially carcinogenic, in food and the resulting food safe-ty has been paid a high attention. Eighty male Sprague-Dawley rats were selected, which were 180-220 g in body weight and 50 d old, and divided into four groups randomly to cunduct the contamination experiment by Acr. The con-taminating dosages were 0, 25, 50, 100 mg·kg^-1 for control and low, middle and high groups, respectively. Intrap- eritoneal injection with Acr for experimental groups and physiological saline for control was done every other day for 12 weeks. Rats in each group were killed and sampled after 4 h since the last contaminating in 3 w, 6 w, 9 w, 12 w. The effect of Acr on the activity of cytochrome P4502E1 in liver were studyed by testing the P4502E1 activity in liver micro-some. The results showed that the activity in 25 mg·kg^-1 group increased with exposure time and the activity in 12 w were obviously higher than that before this time. The P4502E1 activities in 50 and 100 mg·kg^-1 groups peaked in the 6th week . This study on acrylamide metabolism and P4502E1 activity in liver had laid the foundation for further dis-cussing the pathway and toxicity of Acr.
作者 张海明 张丽
出处 《山西农业大学学报(自然科学版)》 CAS 2012年第6期544-547,共4页 Journal of Shanxi Agricultural University(Natural Science Edition)
关键词 丙烯酰胺 环丙氧酰胺 细胞色素P4502E1 Acrylamide (Acr) Glycidamide (GA) Cytochrome P4502E1
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