摘要
目的:观察人参皂苷Rg5对脓毒血症大鼠引起急性肺损伤(ALI)的作用。方法:30只大鼠建立盲肠结扎穿孔法(CLP)脓毒症模型,随机均分为以下3组(n=10):阴性对照组(Sham组)、CLP组及CLP+Rg5组。各组分别于手术后24 h时检测大鼠血清TNF-、IL-6、高迁移率族蛋白1(HMGB1)及IL-10含量;手术24 h后检测肺组织干湿重比(W/D)、髓过氧化物酶活性(MPO)及NF-κB活性;HE法进行肺组织学评分。结果:CLP+Rg5组血浆及肺组织HMGB1和TNF-水平明显低于CLP组(P<0.05)。CLP+Rg5组肺组织W/D、MPO活性、NF-κB活性及肺组织损伤评分均明显低于CLP组(P<0.05)。IL-10水平24 h时在各组动物比较结果差异无统计学意义(P>0.05)。结论:人参皂苷Rg5可以改善脓毒血症引起ALI时炎症反应从而发挥肺保护作用。
Objective： To investigate the effect of ginsenoside Rg5 on acute lung injury in a rat model of sepsis. Methods： The model was established by cecal ligation and puncture （CLP）, and thirty adult male Spragne-Dawley rats were randomly divided into the following groups： Sham group, CLP group, and CLP ＋ Rg5 group （n = 10 for each group）. Serum levels of TNF-, HMGB1 and IL-10 were determined 24 h after operation. Lung was removed for the determinations of pulmonary wet-to-dry ratio （W/D）, myeloperoxidase activity （MPO）, total histological scores, and NF-κB activation at the end of the experiment. Results： Serum levels of HMGB1 and TNF- in CLP ＋ Rg5 group were significantly lower than those in CLP group. There were significantly lower pulmonary levels for W/D, MPO, NF-κB activities, and total histological scores in CLP ＋ Rg5 group than those in CLP group （P 〈 0.05）. There was no difference in serum levels of IL-10 between the groups at 24 h after operation （P 〉 0.05）. Conclusion： Ginsenoside Rg5 attenuated the inflammatory responses, and thus improved ALI in a rat model of sepsis.
出处
《现代生物医学进展》
CAS
2013年第11期2062-2064,共3页
Progress in Modern Biomedicine
基金
江苏省"六大人才高峰"课题(2008093)
