摘要
目的通过测定 ＨＢｅＡｇ阳性慢性肝炎（ＣＨＢ）血清 ＨＢＶ－ ＤＮＡ水平，判断干扰素在病情治疗中的作用。方法 ２８例ＨＢｅＡｇ阳性 ＣＨＢ接受干扰素治疗。血清 ＨＢＶ－ ＤＮＡ浓度测定应用 ＡＣ－９６００ Ａｍｐｌｉｓｅｎｓｏｒ荧光 ＰＣＲ定量系统，测定范围定为１０３００－９５０ｃｏｐｉｅｓ／ｍＬ。结果 ２８例 ＨＢｅＡｇ阳性 ＣＨＢ患者治疗结束时呈完全应答（ＣＲ）者 １３例，无应答（ＮＲ）１５例。对 １３例 ＣＲ患者继续随访６个月，其中４例血清ＡＬＴ再升高（复燃）。血清ＨＢＶ－ＤＮＡ水平在治疗前，ＣＲ不伴ＡＬＴ复燃组、ＣＲ伴ＡＬＴ复燃组和ＮＲ组之间无差异；治疗结束时，ＣＲ不伴 ＡＬＴ复燃组 ＨＢＶ－ ＤＮＡ为 １０３４４ ０４３ｃｏｐｉｅｓ／ｍＬ，明显低于 ＣＲ伴 ＡＬＴ复燃组（１０６８４ ０５１ｃｏｐｉｅｓ／ｍＬ）和ＮＲ组（１０７１８ ０６６ｃｏｐｉｅｓ／ｍＬ）。结论在ＨＢｅＡｇ阳性ＣＨＢ病例，干扰素治疗结束时，检测ＨＢＶ－ＤＮＡ水平对于判断疗效有指导价值，ＨＢＶ－ ＤＮＡ低于 １０４ｃｏｐｉｅｓ／ｍＬ往往能取得持久疗效。
Guo Guangzhou; Xu Kecheng; Ye Ping; et al. (Molecular biology laboratory Luohu people's hospital , Shen zhen 518001) Abstracg:Aim To measure serum hepatitis B virus(HBV)-DNA levels in HBeAg positive patients, and estimate the efficacy of interferon in treating chronic hepatitis B. Methods Twenty eight HBeAg-positive CHB patients were treated with interferon. Quantitative analysis of HBV-DNA was carried out using AG-9600 PCR system. The range of the measurement was 10 3-9.5 copies of genome/mL in serum. Results Among patients heated with interferon 13 eases had complete response(CR), and 15 no response(NR). Among 13 CR, a serum ALT flare-up during 6 months follow-up was observed in 4 patients. Before treatment , serum HBV-DNA in CR without ALT flare-up, CR with ALT flare-up and NR were similar. At the end of treatment serum HBV-DNA in CR without ALT flare-up( copies/mL)was significantly lower than that in CR with ALT flare-up (copies/mL)and NR(copies/mL). Conclusions Measurement of serum HBV--DNA levels is helpful for differentiation of various HBV infectious conditions and for prediction of interferon efficacy in HBeAg-positive chronic CHB pa- tients. Serum HBV-DNA below 104 copies/mL is an important marker for successful heatment.
出处
《胃肠病学和肝病学杂志》
CAS
2000年第3期214-215,共2页
Chinese Journal of Gastroenterology and Hepatology
关键词
乙型肝炎病毒
乙型肝炎
HBV-DNA
干扰素
PCR
Hepatitis B virus Interferons Hepatitis B virus-DNA Polymerase chain reaction Chronic hepatiti
