摘要
【摘要】 目的 探讨金黄色葡萄球菌的耐药性和耐甲氧西林金黄色葡萄球菌的SCCmec基因分型情况。方法 采取纸片琼脂扩散法对500株金黄色葡萄球菌进行药敏检测和耐甲氧西林金黄色葡萄球菌的测定,并采取多聚酶链反应（PCR）法进行SCCmec基因分型检测。结果 耐甲氧西林金黄色葡萄球菌检出率为40.0%（200/500）,而且对克林霉素和复方磺胺甲噁唑以及四环素具有较高的耐药率,其中对庆大霉素和喹诺酮类药物的耐药率较低,同时对克林霉素和β-内酰胺类的药物呈现完全耐药,其他的则表现多重的耐药性,并未发现有耐万古霉素的菌株;通过对MRSA的SCCmec基因分型,其中主要表现为SCCmecⅢ型和 SCCmecⅣ型以及SCCmecⅤ型,另外有7株未分型。结论 在金黄色葡萄球菌的分离中耐甲氧西林金黄色葡萄球菌表现多重耐药性,其SCCmec基因分型主要表现为SCCmecⅢ型和SCCmecⅣ型以及SCCmecⅤ型。
Objective To explore the characteristics of drug resistance of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus SCCmec genotyping.Methods The disc agar diffusion method was taken to test 500 Staphylococcus aureus susceptibility and methicillin-resistant Staphylococcus aureus by multiplex PCR SCCmec genotyping assay.Results The rate of methicillin-resistant Staphylococcus aureus was 40.0%（200/500）,and had a high resistance rate to clindamycin,sulfamethoxazole and tetracycline,which celebrate neomycin and quinolones resist ance rates,while a completely resistant to clindamycin and β-lactam drug,and other performance of multi-drug resistance,resistance to vancomycin had not uncovered any prime strains;through SCCmec genotyping of MRSA,which mainly SCCmec Ⅲ and SCCmec Ⅳ well SCCmec Ⅴ type,another seven unclassified.Conclusion The separation of Staphylococcus aureus,methicillin-resistant Staphylococcus aureus performance of multiple drug resistance performance of its SCCmec genotyping is SCCmec Ⅲ type and SCCmec Ⅳ and SCCmec Ⅴ type.
出处
《中国基层医药》
CAS
2013年第10期1455-1457,共3页
Chinese Journal of Primary Medicine and Pharmacy
基金
河北省唐山市科技计划项目（08130229D）
