摘要
重组蛋白表达在现代生物学技术发展中起着重要的推动作用。通过利用宿主实现外源性蛋白表达,为一些重要蛋白尤其是人源蛋白的结构和功能研究的提供了强有力的工具。促溶标签的应用可以有效地解决重组蛋白表达时常遇到的溶解性问题,并且这些标签还能够对重组蛋白产量、结构和纯化等有一定影响。该综述介绍了几种常见的促溶标签的特性,对其各自的应用特点进行总结,归纳促溶标签最新研究进展。根据文献报导就应用时存在的一些问题和进行探讨,最后展望了促溶标签研究可能产生突破的方面,以便研究者在实验中选择合适的标签。
The expression of recombinant proteins plays an important role in promoting the development of modem biological technology. A powerful tool for structural and functional studies of important proteins, especially proteins of human beings, could be provided by produ- cing heterologous proteins in the hosts. The application of solubility - enhancing tags could overcome the soluble problem in expressing re- combinant proteins. And, the yield, structure modification and purification of recombinant proteins could be influenced by the fusion part- ners. In the present review, the characteristics of several common solubility enhancers were introduced. The recent advances on the solubil- ity -enhancing fusion tags were showed and the potential problems in their application were discussed. At last, the future was prospec- ted. This review might be helpful for researchers in choosing suitable tags in their experiments.
出处
《生物技术》
CAS
CSCD
北大核心
2013年第2期93-97,共5页
Biotechnology
关键词
融合表达
促溶标签
重组蛋白
应用
fusion expression
solubility, enhancing tag
recombinant protein
application
