摘要
目的探讨炎症性肠病(inflammatory bowel disease,IBD)湿热转化不同证型的免疫学基础。方法采用综合因素(饮食加气候环境加药物加致病生物因子)造模复制IBD湿热证动物模型。于造模完成后分别检测TNF-α、白细胞介素(IL-1β、IL-4、IL-6、IL-10)和淋巴T细胞抗体(CD3+、CD4+、CD8+)。结果造模在发病条件、主要症状和体征方面近似IBD湿热证型。实验组血清TNF-α、IL-1β、IL-6均低于对照组(P<0.05),IBD模型组与对照组的血清IL-4、IL-10和CD4+抗原比较均无统计学意义(P>0.05)。结论 TNF-α、IL-1β、IL-6参与了IBD湿热证的发病过程,IBD的免疫过程是一个非特异性免疫过程。
Objective To study,the immunological basis of damp-heat conversion of different syndromes of inflammatory bowel disease （IBD）. Methods Animal models of inflammatory bowel disease damp-heat syndrome were developed by using various factors including diet, climate, drugs, and pathogenic biological factors. TNF-o~, interleukins （ IL-113, IL-4, IL-6, and IL-10） and lymphoid T cell antibodies （CD3＋ , CIM＋ , CD8＋） were measured after the models were completed. Results The body temperature of animals with IBD damp-heat syndromes was different from that of normal animals. Serum levels of TNF-c~, IL-113 and IL-6 of the experimental group were significantly lower than that of the Control group （P 〈 0. 05 ）. However, no significant difference in serum levels of IL-4 and IL-10, and numbers of CIM~ T cells were found between the IBD groups and the control group. Conclusion TNF-ct,IL-113,IL-6 may be in- volved in pathogenesis of IBD with damp-heat syndromes. The immune response of IBD with damp-heat syndrome is a non-specific im- mune process.
出处
《实用医院临床杂志》
2013年第3期50-53,共4页
Practical Journal of Clinical Medicine
关键词
炎症性肠病
湿热转化
动物模型
免疫细胞因子
Inflammatory bowel disease
Damp-heat conversion
Animal models
Immune cytokines
