摘要
目的从束缚强度、高脂饮食、血糖测定等方面探索慢性束缚应激模型用于观测高血糖相关指标的方法。方法实验SD大鼠施加为期4个周期、每周期10d、每天12h的慢性束缚应激,喂饲普通或高脂饲料,并设高脂+链脲佐菌素(STZ)组、单纯高脂组和正常大鼠为对照。每个束缚周期结束后第3d用针刺大鼠尾静脉取血,血糖仪监测各组大鼠血糖;最后一个束缚周期结束后第3d处死大鼠,ELISA检测血清胰岛素、肾上腺素和皮质酮水平;胰腺行HE染色及胰岛素、胰高血糖素免疫组织化学检测。结果采用慢性束缚应激成功制备高血糖动物模型,成模率为32.5%;慢性束缚应激成模大鼠血清胰岛素水平高于3个对照组(P<0.05),而高脂+STZ组及单纯高脂饮食大鼠血清胰岛素水平与正常对照组比较差异无统计学意义;胰腺HE染色及免疫组化显示慢性束缚应激大鼠胰腺体积增大,胰岛素水平下降(P<0.05);高脂因素可引起胰岛素和胰高血糖素水平均下降(P<0.05);高脂+STZ大鼠胰岛严重破坏,未见胰岛素、胰高血糖素表达。各实验组大鼠血清肾上腺素、皮质酮水平与正常对照组比较差异均无统计学意义。结论在本实验造模条件下可成功制备慢性束缚应激高血糖大鼠模型,相关指标检测方法具有可靠性。
Objective To explore chronic constraint stress （CCS） animal model in the application to observe hyperglycemia by improving the method of constraint, diet food and blood glucose assay. Methods Chronic constraint stress was performed 12 h per day on SD rats for 4 periods with 10 d per periods. The rats were fed normal diet （ND） or high fat diet （HFD） respectively, while HFD＋ Streptozotocin （STZ） and HFD as control. Blood glucose was tested on the 3rd day after every bound period. Insulin, cortisone and adrenaline in serum were tested by ELISA at 3 days after the last bound period. The expressions of insulin and glucagon in pancreas were detected by immunohisto-chemical （ICH） straining. Results There were 32.5% rats suffering hyperglycemia after chronic constraint stress. Compared with control group, the serum level of insulin increased significantly in CCS group. The blood glucose and serum insulin levels in the HFD-STZ and HFD group were not significantly different to those of control group （P〉0.05）. HE and ICH sections showed that the pancreas volume of CCS rats increased, insulin expression in islets decreased. Insulin and glucagon expression in HFD islets decreased. Islets of STZ rats were seriously damaged and no insulin or glueagon positive cells were observed. Conclusion CCS could induce hyperglycemia, which might be used in the further study on type 2 diabetes mellitus.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2013年第3期470-475,共6页
Journal of Sichuan University(Medical Sciences)
关键词
慢性束缚应激
链脲佐菌素
高血糖
动物模型
Chronic constraint stress Streptozotocin Hyperglycemia Animal model
