摘要
目的:观察奥扎格雷钠对实验性心肌缺血大鼠心肌组织NO及eNOS的影响。方法:将心电图正常的健康大鼠随机分成空白对照组(0.15mL生理盐水)、模型对照组(0.15mL生理盐水)、低剂量组(8g/kg奥扎格雷钠)、中剂量组(12g/kg奥扎格雷钠)、高剂量组(16g/kg奥扎格雷钠)和阳性对照组(81.0mg/kg复方丹参滴丸),连续灌胃给药7d,从第3d起除空白对照组外,其余各组均于给药后1h皮下注射异丙肾上腺素5mg/kg.d,连续5d,时间间隔为24h,正常对照组给予等量生理盐水皮下注射,末次注射异丙肾上腺素2h后取心肌组织测定NO、eNOS的活性。结果:与空白对照组相比,模型对照组大鼠的eNOS活力、总NOS活力明显提高,NO浓度明显降低(P<0.05);与模型对照组相比,低剂量组大鼠eNOS活力、总NOS活力降低,NO浓度提高(P>0.05),差异不显著,无统计学意义,而中剂量组及高剂量组、阳性对照组大鼠血清eNOS活力、总NOS活力明显减轻,NO浓度明显提高(P<0.05);中剂量组及高剂量组与阳性对照组比较,eNOS活力、总NOS活力降低程度,NO浓度提高程度相当。结论:奥扎格雷钠能有效地升高心肌组织NO浓度,抑制eNOS及总NOS活力系数,清除自由基,减轻心肌损伤。
Objective： To explore the influence of ozagrel sodium on NO and eNOS in myocardial tissue of myocardial ischemia rat. Methods： The electrocardiogram normal and health Wistar rats were randomly divided into control group （0.15 mL saline）, model group （0.15 mL saline）, low dose group （8g/kg ozagrel sodium）, middle dose group （12 g/kg ozagrel sodium）, high dose group （16 g/kg ozagrel sodium） and positive control group （81.0 mg/kg nitroglycerin）. They had continuous intragastric administration for 7 day. from the third day, in addition to the normal control group, the other groups had isoproterenol subcutaneous injection at 5 mg/kg · d -1 hour after administration for 5 days continuously, with interval time as 24 h. The normal control group received normal saline by subcutaneous injection. NO, eNOS of myocardial tissue were determined 2 hour after the injection of isoprenaline. Results：Compared with the control group, eNOS activity and total NOS activity were significantly increased, NO concentration was decreased significantly in model group （P〈0.05）; Compared with model group, eNOS activity and total NOS activity were decreased, and NO concentration was increased in low-dosage group （P〈 0.05）. eNOS activity and total NOS activity were significantly decreased, and NO concentration was significantly increased in middle dose group, high dose group, and positive control group （P〈0. 05）. Decrease in NOS activity and NOS activity was more significant in middle dose group and high dose group, and increase in NO concentration was not significant. Conclusions： Ozagrel sodium can effectively increase NO concentration in myocardial tissue, inhibit activity coefficient of eNOS and NOS, scavenge free radicals, and reduce myocardial injury.
出处
《海南医学院学报》
CAS
2013年第5期593-595,599,共4页
Journal of Hainan Medical University
基金
中国高校医学期刊临床专项资金项目(112210624)~~
