期刊文献+

Plackett-Burman试验设计联合星点设计效应面法优选蜘蛛香中总缬草三酯提取工艺 被引量:4

Optimization of Extraction Technology for Total Valepotriates from Valeriana jatamansi by Plackett-Burman Design Combined with Central Composite Design and Response Surface Methodology
原文传递
导出
摘要 目的:采用Plackett-Burman试验联合星点设计效应面法优选蜘蛛香中总缬草三酯的提取工艺。方法:在单因素试验基础上,通过Plackett-Burman试验筛选主要影响因素;采用星点设计-效应面积法优选蜘蛛香中总缬草三酯提取工艺,以乙醇体积分数、浸泡时间、超声时间为自变量,总缬草三酯含量为因变量,通过对自变量与因变量的完全二次响应曲面的回归拟合,利用三维曲面图直观分析总缬草三酯的最佳提取工艺,并进行预测分析。结果:最佳提取工艺为乙醇体积分数65%,液料比10∶1,浸泡时间3 h,室温超声2次,每次30 min。结论:优选的提取工艺简便、精度更高、重复性好、预测性强。 Objective: To optimize extraction process of total valepotriates from Valeriana jatamansi by Plackett-Burman combined with central composite design-response surface methodology. Method: Based on single factor test,Plackett-Burman test was adopted to screen main influencing factors;Extraction technology of total valepotriates was optimized by central composite design-response surface method,with the concentration of ethanol,soaking time,ultrasound time as independent variables,dependent variable was the content of total valepotriates,through complete quadratic response surface regression fitting of independent variables and the dependent variable,optimum technology was determined by intuitive analysis of 3D surface chart,and predictive analysis was used. Result: Optimum extraction technology was as following:the concentration of ethanol 65%,soaking time 3 hours,liquid-solid 10: 1,ultrasonic extracted 2 times at room temperature,30 minutes per time. Conclusion: This optimized process was simple,accurate,reproducible with good predictability.
出处 《中国实验方剂学杂志》 CAS 北大核心 2013年第10期38-41,共4页 Chinese Journal of Experimental Traditional Medical Formulae
基金 湖北省自然科学基金重点项目(2010CDA047)
关键词 蜘蛛香 缬草三酯 PLACKETT-BURMAN 星点设计(CCD) 提取工艺 Valeriana jatamansi total valepotriates Plackett-Burman central composite design extraction process
  • 相关文献

参考文献7

二级参考文献28

  • 1明东升,郭济贤.缬草类生药中总缬草素的含量测定[J].上海医科大学学报,1993,20(3):210-212. 被引量:11
  • 2曹斌,洪庚辛.蜘蛛香的中枢抑制作用[J].中国中药杂志,1994,19(1):40-42. 被引量:18
  • 3陈业高,于丽丽,张燕.马蹄香化学成分的分离与鉴定[J].云南化工,2005,32(5):13-16. 被引量:15
  • 4肖丹,管咏梅,边燕红.蜘蛛香药材中总缬草素的含量测定[J].时珍国医国药,2006,17(2):198-199. 被引量:5
  • 5国家药典委员会.中国药典[S].一部.北京:化学工业出版社,1977:633.
  • 6Bounthanh C,Bergmann C,Beck G P,et al.Valepotriates,a new class of cytotoxic and antitumar agents[J].Planta Med,1981,41(1):21.
  • 7Marderm,Violah,Wasowski C,et al.6-Methylapigeninandhesperidin:Newvaleriana flavonoids with activityonthe CNS[J].Pharm Bio Behav,2003,75(3):537.
  • 8Sebastia'n Ferna'ndez,Cristina Wasowski,Alejandro C Paladini,et al.Sedative and sleep-enhancing properties of linarin,a flavonoid-isolated from Valeriana officinalis[J].Pharm Bio Behav,2004,77:399.
  • 9中国药典,一部[S].2010:263.
  • 10Roberto Andreatini, Vania A Sartori, Maria L V, et al. Effect of Valepotriates (Valerian Extract) in generalized anxiety disorder: a randomized placebo-controlled pilot study [J]. Phytother Res, 2002, 16(7): 650.

共引文献476

同被引文献51

引证文献4

二级引证文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部