摘要
利用ISSR标记技术对83份早熟荔枝(品种)单株遗传多样性进行分析。筛选出多态性高的10条IS-SR引物,共扩增出128条DNA条带,其中多态性带107条,多态性百分率为83.59%,表明桂西南早熟荔枝实生资源遗传多样性较丰富;用NTSYS软件计算出这83份材料的DICE相似系数在0.64～0.95之间,遗传亲缘关系较近;用UPGMA方法构建分子树状图,在相似系数0.75时,可将栽培品种与桂西南早熟实生单株区分开。各地区资源混杂聚类在一起,不能按地区单独聚类。
The genetic diversities of 83 early-maturing litchi（variety） plants were examined by using ISSR markers in this study. Ten primers gave reproducible, polymorphic DNA amplification patterns with a total of 128 amplified bands. The percentage of polymorphic band was 83. 59%, which showed that the early-maturing seedling litchi re- sources in southwest Guangxi had abundant genetic diversity. The genetic similarity ranged from 0.64 to 0.95 based on DICE. A dendrogram was constructed by UPGMA method,and the early-maturing litchi（variety）plants were dis- tinguished from cultivar litchi at a similarity coefficient criterion of 0.75. No significant correlation between the den- drogram and geographical distance was revealed among the resources.
出处
《广西植物》
CAS
CSCD
北大核心
2013年第2期225-228,共4页
Guihaia
基金
国家荔枝龙眼产业技术体系育种岗位“熟期育种”(CARS-33-03)
广西农业科学院基本科研业务专项(桂农科2013YT03)
关键词
早熟荔枝
实生资源
遗传多样性
ISSR
early-maturing litchi
seedling litchi resources
genetic diversity
ISSR markers
