摘要
目的 探讨流式细胞技术测定异基因外周血干细胞移植 (allo PBSCT)中供者细胞表面分化抗原 34 (CD34 + )细胞及其亚群变化和意义。方法 应用流式细胞多色分析技术 ,测定 15 1份allo PBSCT供者 ,经细胞因子动员后外周血标本CD34 + 及其亚群变化及影响因素。结果 在检测的15 1份标本中 ,CD34 + 细胞占外周血单个核细胞的 (0 .95 4± 0 .46 6 ) % ,含量为 (3.5 5± 2 .41)× 10 9/L ;其中CD34 + CD38-亚群含量为 (0 .2 5 3± 0 .2 40 )× 10 9/L ,占CD34 + 细胞的 6 .78% ;CD34 + HLA DR 亚群含量为 (0 .2 73± 0 .310 )× 10 9/L ,占CD34 + 细胞的 6 .82 % ,两者差异无显著意义 (P >0 0 5 ) ;随着采集次数的增加 ,CD34 + 细胞及其亚群数量逐渐减少 (P <0 .0 5 ) ;随着供者年龄增加 ,其外周血CD34 + 细胞数逐渐减少 ,≥ 40岁供者CD34 + 细胞百分比和含量比 <2 0岁供者分别降低了 47%和 5 0 % ;动员后外周血CD34 + 细胞数存在性别差异 ,男性供者外周血CD34 + 细胞数较女性高 2 3%。结论 应用流式细胞多色技术测定外周血造血干祖细胞 ,不仅能确定造血细胞数量 ,而且对造血干祖细胞的质量进行评价 ,为临床干细胞移植治疗提供重要数据。
Objective To detect and analyze CD34 + cells and their subsets of peripheral blood in donor for allo PBSCT by flow cytometry.Methods Flow cytometry and multi color McAbs were used to detect CD34 + cells and their subsets.Results In 151 samples, the percentage of the CD34 + cells in MNCs was (0.954± 0.466)%, and the calculation of absolute CD34 + cells was (3.55±2.41)×10 9/L. The calculation of absolute CD34 +CD38 - cells was (0.253±0.24)×10 9/L, which was 6.78% of CD34 + cells;the calculation of absolute CD34 +HLA DR cells was (0.273±0.310)×10 9/L, or 6.82% of CD34 + cells. There was no significant difference between the enumeration of the CD34 +CD38 - cells and that of the CD34 +HLA DR cells. With the increasing of collection times and the donor's age, the numbers of CD34 + cells decreased accordingly. The percentage and the absolute CD34 + cells decreased by 47% and 50% respectively in donors above 40 years old than in donors below 20. There is a difference between numbers of CD34 + cells male and female donors, 23% higher in men the letter.Conclusion Detecting and analysing CD34 + cells and their subsets by flow cytometry and multi color analysis can not only give us exact numbers of CD34 + cells, but show us the quality of the hematopoietic stem cells for allo PBSCT.
出处
《中华检验医学杂志》
CAS
CSCD
2000年第4期207-210,共4页
Chinese Journal of Laboratory Medicine
