摘要
为了研究G蛋白偶联受体30(GPR30)和神经生长因子(NGF)在发情周期山羊卵巢中的表达规律,运用免疫组织化学SP法分别对发情前期、发情期、发情后期和间情期关中奶山羊卵巢中GPR30和NGF的定位和相对表达量进行了研究。结果表明,GPR30和NGF免疫反应阳性产物不同程度地分布于发情周期山羊卵巢各级卵泡的颗粒细胞、膜细胞、卵母细胞以及黄体细胞和间质细胞;两者在各级卵泡中的表达量不同,GPR30在各级卵泡的相对表达量以发情期极显著高于其他3个时期(P<0.01);NGF在原始卵泡中以发情期和发情前期表达量极显著高于其他2个时期(P<0.01),在初级卵泡以间情期极显著低于其他3个时期(P<0.01),在发情周期的次级卵泡和三级卵泡中的表达规律和GPR30相似,间情期NGF在黄体细胞的表达强度极显著高于发情后期(P<0.01)。表明GPR30和NGF不同程度地参与关中奶山羊卵巢卵泡的发育和成熟以及激素分泌的调节。
In order to study the expression of G protein-coupled receptor 30（GPR30） and nerve growth factor（NGF） in the ovary of goat during the estrous cycle,immunohistochemistry SP method was used to study the distribution and expression of GPR30 and NGF in the ovary of Guanzhong dairy goats in proestrus,estrus,metestrus and diestrus. The results showed that positive GPR30 and NGF staining was located in the granular cells,theca interna cells, oocytes, luteal cells,interstitial cells of the follicles at different levels during the estrous cycle. The expression of GPR30 and NGF in the follicles were different. The expression level of GPR30 in all follicle during the estrus was significantly higher than that of GPR30 during other three periods（P〈0.01）. The protein of NGF was expressed in the ovary throughout the whole estrous cycle. Its expression levels in the primordial follicles during the proestrus and estrus were significantly higher than that during the other two periods（P〈0.01）, while the expression level during the diestrus was significantly lower than that during other periods（P〈0.01）. The expression of NGF in the secondary follicles and tertiary follicles was identical to GPR30. The expression level of NGF in the luteal cell during the diestrus was higher than that during the metestrus（P〈0.01）. This study suggests that GPR30 and NGF play their roles in regulating the estrogen action in development and maturity of follicles and hormone secretion at different levels during the estrous cycle in Guanzhong dairy goats.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2013年第5期522-526,共5页
Chinese Veterinary Science
基金
抗病转基因牛新品种培育重点专项(2009ZX08007)
