摘要
目的建立大黄酚冻干脂质体的制备工艺。方法采用薄膜-超声分散法制备出大黄酚脂质体悬混液,低速离心法分离游离药物测定包封率,考察预冻时间、干燥时间、保护剂类型、保护剂加入方式及其用量对冻干脂质体包封率的影响。结果预冻时间为24 h,干燥时间为24 h,以甘露醇-蔗糖(质量比1∶1)为保护剂外加法得到的大黄酚冻干脂质体粒径均匀,复溶性极好,包封率达(61.30±2.2)%。结论采用薄膜-超声分散法制备出大黄酚脂质体悬混液,以甘露醇-蔗糖作保护剂,可制得复溶性极好、包封率较高的大黄酚冻干脂质体。
Objective To establish a method for the preparation of chrysophanol freeze-dried liposomes. Methods The liposome suspension was prepared by film-ultrasonic dispersion method. The entrapment efficiency （EE） was determined by low-speed centrifugation and the influences of pre-freezing time, drying time, and type, dosage, and adding methods of the protective agents on EE of the freeze-dried liposome were studied. Results The pre-freezing time was 24 h, the drying time was 24 h, mannitol-sucrose （mass ratio 1 ： 1） as protective agent was externally added to get uniform particle with good redissolution and the EE was （61.30 ±2.2）%. Conclusion The liposome prepared by film-ultrasonic dispersion method is protected by mannitol-sucrose to get uniform particle size with the excellent redissolution and high EE.
出处
《中草药》
CAS
CSCD
北大核心
2013年第8期960-964,共5页
Chinese Traditional and Herbal Drugs
基金
河北省科学技术研究与发展计划项目(12276104D-94)
河北北方学院自然科学青年基金项目(Q201112)
关键词
大黄酚
冻干脂质体
薄膜-超声分散法
低速离心法
包封率
chrysophanol
freeze-dried liposomes
film-ultrasonic dispersion method
low-speed centrifugation
entrapment efficiency
