摘要
应用PCR-DGGE技术研究4℃冷藏期间牡蛎鳃部菌群的动态变化及腐败菌群.直接从牡蛎鳃部中提取细菌总DNA,对细菌的16SrDNA的V3区进行PCR扩增,扩增产物进行变性梯度凝胶电泳(DGGE),分析DGGE微生物指纹图谱.DGGE指纹图谱结果表明,新鲜牡蛎鳃部菌群复杂,随冷藏期的变化菌条带变化明显;整个冷藏期间Lactococcus raffinolactis和Enterobacter sp.为牡蛎鳃部的优势菌,牡蛎冷藏后期腐败的主要菌群为假单胞菌属(Pseudomonas sp.)、气单胞菌属(Aeromonas spp.)、乳球菌属(Lactococcus spp.)、肠杆菌属(Enterobacter spp.)、Weissella confusa和Lactobacillus sakei.
PCR-DGGE technology has been used to reveal the dynamics for microbial flora and dominant spoilage bacteria in oyster gills during storage at 4 ℃. Total bacterial genome DNA has been extracted di reetly from oyster gill tissues and the V3 region of the 16S rDNA have been amplified. The amplicons have been analysis by DGGE fingerprinting. Results show that microbial flora can be complicated in fresh oyster gills and the bands can change significantly during refrigeration storage. It has been found that, in the whole refrigeration period, Lactococcus raffinolactis and Enterobacter sp. can be regarded as dominant bacteria for oyster gill. The predominant spoilage bacteria in oyster gill has been found to be Pseudomonas sp. , Aeromonas spp. , Lactococcus spp. , Enterococcus spp. , Weissella confuse, and Lactobacillus sakei at the later period of refrigeration.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第4期151-156,共6页
Journal of Southwest University(Natural Science Edition)
基金
福建省自然科学基金资助项目(2009J01234
2012J01092)
福建省教育厅科技项目(JA07197)
关键词
牡蛎鳃部
PCR
DGGE
菌群
腐败
oyster gill tissue
PCR-DGGE
microbial flora
spoilage
