摘要
目的:探讨前胡丙素对自发性高血压大鼠(SHR)心肌重构的影响及可能机制。方法:SHR 20只,完全随机分为药物组[前胡丙素20 mg/(kg.d)灌胃,n=10],对照组(n=10),另设SD大鼠10只为正常组,干预8周,计算心脏质量指数及左室质量指数,光镜观察左心室心肌细胞形态和结构的变化,透射电镜观察心肌细胞超微结构及线粒体损伤情况,然后取左心室心肌制备匀浆,提取RNA,用半定量RT-PCR法测定受磷蛋白的基因表达水平,用液体闪烁计数法测定受磷蛋白的磷酸化水平。结果:与对照组比较,药物组用前胡丙素治疗8周后,心脏及左室质量指数明显下降(P<0.05),SHR心肌肥厚等病理改变明显改善,左心室心肌受磷蛋白mRNA表达上调(P<0.05),心肌受磷蛋白磷酸化水平增加(P<0.05),差异具有统计学意义。结论:前胡丙素有改善心肌重构作用,可能是通过上调SHR心肌肌浆网受磷蛋白表达及磷酸化水平而实现的。
Objective：To explore the impact and possible mechanism of praeruptorin C on cardiac hypertrophy in spontaneously hypertensive rats（SHR） and its possible mechanisms. Methods：Totally 20 SHR were randomly divided into the praeruptorin C group [praeruptorin C 20 mg/（kg·d）intragastric administrated,n=10]and the untreated control group （n = 10）,with 10 age matched Sprague Dawley rats as normal group. The rats of each group were intervened for 8 weeks. We calculated the heart quality mass index （MHI） and left ventricular mass index（LVMI）. We observed the morphological and structural changes of cardiomyocytes with optical microscope,and then the injuries of cell ultrastructure and mitochondria with transmission electron microscope. Then,we made homogenate and extracted RNA taken from heart tissue. The gene expression levels of pbospholamban were determined by the method of semiquantitative RT-PCR. The phosphorylation of PLB was counted by liquid scintillation. Results：Compared with the SHR control group, the LVMI and HMI of SHR treated with praeruptorin C for 8 weeks were decreased （P 〈 0.05）,the cardiac hypertrophy were reversed, the levels of PLB mRNA expression and the phosphorylation of PLB of the rats in the praeruptorin C group were increased （P 〈 0.05）. Conclusion：Praeruptorin C had a certain effect on reversing the cardiac hypertrophy. The possible mechanisms might be upregulating the gene expression of sarcoplasmic reticulum phospholamban and increasing the phosphorylation level.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第4期467-470,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
连云港市重点人才课题(Zc229)
关键词
前胡丙素
自发性高血压大鼠
受磷蛋白
磷酸化
praeruptorin C
spontaneously hypertensive rats
phospholamban
phosphorylation
