摘要
目的:探讨不同浓度的5-氮杂胞苷对RPMI 8226细胞系的诱导凋亡作用。方法:对RPMI 8226细胞系采用5-氮杂胞苷0μmol/L、2μmol/L、5μmol/L、10μmol/L、20μmol/L、50μmol/L处理24 h、48 h、72 h,并对RPMI 8226细胞系处理后进行刮痕实验,12 h后对细胞迁移进行比较。结果:在作用24h、48h时,随着作用浓度的增加,RPMI-8226细胞的抑制率出现明显的增加,但在作用72 h时,我们发现10μmol/L、20μmol/L、50μmol/L其对RPMI-8226细胞的抑制效果无明显的差异性;刮痕实验后12 h后其出现差异性,其中药物浓度越大其对RPMI-8226细胞的运动迁移能力越弱。结论:DNA甲基化转移酶抑制剂5-氮杂胞苷可对RPMI-8226细胞的凋亡具有良好的诱导效果,同时可抑制RPMI-8226细胞的增殖以及迁移。
Objective： To explore the induction of different concentrations of 5-azacytidine to RPMI 8226 cell lines apoptosis.Methods： The RPMI 8226 cell lines were treated by the 5-azacytidine of different concentrations（0 μmol /L,2 μmol /L,5 μmol /L,10 μmol /L,20 μmol /L,50 μmol /L） for 24 h,48 h,72 h.After treatment,the RPMI 8226 cell lines were processed with scratches experiment,then the cell migration was compared at 12 h later.Results： After treated for 24 h or 48 h,RPMI-8226 cell inhibition rate increased significantly along with the increased concentrations,but after 72 h,we found that the 10 μmol /L,20 μmol /L,50 μmol /L of 5-azacytidine had no significant difference in the inhibitory effect on RPMI-8226 cells.12 h after scratches experiment appeared difference,the higher the concentration of the drug,the weaker movement migration capability of RPMI-8226 cells.Conclusion： DNA methyltransferase inhibitor 5-azacytidine had good induction on RPMI-8226 cell apoptosis,and inhibited cell proliferation and migration of RPMI-8226.
出处
《现代生物医学进展》
CAS
2013年第13期2437-2439,2444,共4页
Progress in Modern Biomedicine
