摘要
目的 了解玻璃体内注射氟康唑对眼内组织的毒性作用 ,探索眼内应用氟康唑的安全剂量。 方法 12只健康成年大耳白兔随机分为 6组 ,一组为对照组 ,其余 5组右眼玻璃体内分别注射 10、5 0、10 0、15 0、2 0 0 μg氟康唑。以检眼镜、视网膜电流图、光镜和电镜观察注药 3、14d后药物对视网膜的毒性。 结果 对照组及氟康唑 10～ 15 0 μg组注药 3、14d后视网膜各层细胞结构大致正常。 2 0 0 μg组注药 14d后光镜及电镜均显示视网膜各层细胞呈明显变性改变。光镜显示外节空泡变性 ,外核层细胞核外移至内节 ,神经纤维层空泡变性 ,色素上皮增生。电镜显示双极细胞核周池扩张、核固缩 ,内丛状层神经突起肿胀、突触消失 ,色素上皮细胞空泡化改变、微绒毛消失。 结论 氟康唑玻璃体内注射的安全剂量以 10 0～ 15 0 μg为宜。
Objective [WT5”BZ]To investigate the retinal toxicity and verify the safe dose of intravitreal injecting fluconazole. [WT5”HZ]Methods [WT5”BZ]Twelve healthy adult white rabbits were divided at random into 6 groups:a normal control group and 5 groups received intravitreal injection of a single dose of fluconazole ranging from 10 to 200 μg respectively.Retinal toxicity was examined by ophthalmoscopy, electroretinography, light and transmission electron microscopy (TEM) on the third and fourteenth day after injection. [WT5”HZ]Results [WT5”BZ]The ultrastructures of the retinal tissues of the normal control group and fluconazole 10～150 μg groups were normal on the third and fourteen day after injection.The light microscopy and TEM showed that cells of all the retinal layers in the 200 μg group revealed apparent degenerative changes on the fourteenth day after injection, and the light microscopic picture showed the vacuolar degeneration of outer segments of photoreceptors, the nuclei in outer nuclear layer drop out into inner segments, the vacuolar degeneration of nerve fiber layer, and the proliferation of pigment epithelium. TEM revealed expansion of paranucleus space and karyopyknosis of the bipolar cells, the swelling of nerve fibers and disappearance of the synapses in the inner plexiform layer, the vacuolation and disappearance of microvilli of the pigment epithelium cells. [WT5”HZ]Conclusion [WT5”BZ]The safe dose of fluconazole injected intravitreally should be 100～150 μg. [WT5”HZ]
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
2000年第3期201-203,共3页
Chinese Journal of Ocular Fundus Diseases
关键词
氟康唑
眼毒性
玻璃体内注射
安全剂量
Fluconazole/toxicity
Fluconazole/administration dosage
Retina/drug effects
Vitreous body
