摘要
目的了解铜绿假单胞菌的来源分布及其对12种抗菌药物的耐药性,为临床合理治疗提供指导。方法对2011年1-12月送检标本进行常规的分离培养,采用VITEK-2Compact GN卡或API 20NE板条进行细菌鉴定,并用K-B法进行药敏试验。结果共检出铜绿假单胞菌303株,其中ICU检出率最高为26.7%,以痰标本的分离率最高为58.1%,其次为伤口分泌物27.0%;铜绿假单胞菌对哌拉西林/他唑巴坦的敏感率最高为83.2%,对米诺环素、亚胺培南的敏感率最低,在20.2%～43.6%;ICU敏感率均低于其他病区,其中头孢他啶、亚胺培南差异有统计学意义(P<0.05);共检出泛耐药铜绿假单胞菌22株,主要来自ICU占68.2%。结论 ICU铜绿假单胞菌耐药现象严重;应加强对铜绿假单胞菌的耐药性监测以指导临床合理用药,同时应采取有力措施防止耐药菌株蔓延。
OBJECTIVE To understand the clinical distribution of specimen sources and drug resistance of 12 antibiotics against 303 strains of PseudOmonas aeruginosa so as to guide the reasonable clinical treatment. METHODS The specimens were collected to perform the bacterial isolation and culture from Jan to Dec, 2011. The VITEK 2 compact GN cards or API 20NE kits were used to identify the strains. The drug susceptibility testing wos performed by K-B methods. RESULTS A total of 303 strains of P. aeruginosa were isolated. The isolation rate of P. aeruginosa from the intensive care unit （ICU） was the highest （26. 7%）. The majority of the P. aeruginosa strains were isolated from the sputum （58. 1%）, followed by wound secretions （27. 0%）. The susceptibility rate to piperacillin/tazobactam was the highest（83.2 %）. However, the drug susceptibility rates to minocycline, and imipenem were the lowest, varying between 20.2 % and 43.6 %. The susceptibility rate of the P. aeruginosa strains from ICU was lower than the strains from other wards . There was statistical difference in the susceptibility rate between the ceftazidine and imipenem （P〈0.05）. Totally 22 strains of pandrug-resistant P. aeruginosa were isolated, 68.2% of which were isolated from the ICU. CONCLUSION The drug resistance of the P. aeruginosa strains from the ICU is increasingly severe. It is necessary to strengthen the monitoring of the drug resistance to guide the reasonable clinical medication and to take effective measures to prevent the spread of drug resistant strains.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2013年第10期2464-2466,共3页
Chinese Journal of Nosocomiology
