摘要
目的探讨一种大鼠脑组织石蜡切片免疫组化切实可行的技术方案,并得到满意的实验结果。方法改进石蜡切片制作过程,以大鼠海马神经元caspase-3染色为例,按SABC法进行免疫组化染色。结果组织切片完整均匀,海马区组织、细胞形态正常,未见扩张的毛细血管,可清晰见到细胞质和/或核呈棕黄色的阳性细胞,细胞核呈紫蓝色的正常细胞,背景染色少。结论通过对大鼠脑组织取材、脱水、以及采用合理抗原修复液等方面的改进,可以较好解决大鼠脑组织免疫组化染色中出现的问题,提高免疫组化染色质量。
Objective To explore a practical technical process of immunohistochemistry on the paraffin sections of rat brain tissues for obtaining the satisfactory results. Methods The caspase-3 staining in rat hippocampal neurons was done according to SABC method after improving the section-preparing process. Results Tissue sections were complete and uniform, the hippocampus tissue and cell morphology were normal. The expansion of the capillaries was not seen. The positive cells that cytoplasm and/or nuclear brown are yellow and the normal cells that nuclei are purple and blue were clearly seen, and the background staining was less. Conclusion The improvement on rat brain tissue selected, dehydration, and reasonable antigen retrieval solution in the experiments could improve the aualitv of immunohistochemieal staining,.
出处
《山西医科大学学报》
CAS
2013年第4期311-312,328,共3页
Journal of Shanxi Medical University
基金
山西省科技攻关基金资助项目(20110313011-1)
山西省高等学校科技基金资助项目(2010109)
关键词
脑组织
石蜡切片
免疫组化
brain tissue
paraffin sections
immunohistochemistry
