摘要
目的:探讨丝裂原活化蛋白激酶p38(p38MAPK)在肺鳞癌组织中的表达及意义。方法:采用real-time PCR检测肺鳞癌(lung squamous cell carcinoma,LSCC)患者癌组织和癌旁正常肺组织(non-tumor adjacenttissues,NAT)中p38MAPK mRNA表达水平;Western blot法检测p38MAPK(p38)和磷酸化p38MAPK(P-p38)蛋白表达水平,免疫组织化学法检测p38和P-p38在肺鳞癌组织中的定位。应用SPSS12.0软件分析与临床病理参数的关系。结果:肺鳞癌组织中p38MAPK mRNA和蛋白表达水平与对照组间均无明显差异(P>0.05)。癌旁正常肺组织中P-p38蛋白表达为(0.14±0.03),肺鳞癌组织中为(0.45±0.04),差异有统计学意义(P<0.05);P-p38蛋白表达与和肺鳞癌的组织分级程度呈正相关,与临床分期呈负相关(P<0.05)。结论:p38MAPK只有经过磷酸化活化后才发挥功能,即P-p38。P-p38可能在肺鳞癌的发生和发展中起到抑制作用,可以作为肺鳞癌的早期诊断和治疗的参考指标。
Objective：To study the expression and its significance of p38MAPK in human lung squamous cell carcinoma（LSCC）. Methods ：To investigate p38MAPK expression in LSCC,65 patients were enrolled and tumor tissues were collected with matched non - tumor adjacent tissues（NAT）, p38MAPK mRNA and protein levels were quantified with real- time PCR, Western blots and immunohistochemistry. Moreover, the distribution of p3 8 and P- p38 were further confirmed by 10 paired of formalin - fixed paraffin - embedded（FFPE） LSCC and NAT by immunohistochemistry. SPSS12.0 was used to analyze the relationship between the p38MAPK expression and clinicopathological significance. Results：p38MAPK mRNA and protein levels were quantified with real- time PCR and Western blots, The p38MAPK expression did not differ between tumor tissue and controls at mRNA levels and protein（P 〉0.05）. However,P -p38 was significantly lower expressed in LSCC compared to NAT. The expression of P -p38 was negatively correlated with the clinical stage and was positively associated with histodifferentiation（P 〈 0.05 ）. Conclusion： P- p38 may play very important role in inhibitory effects during the occurring and developing of LSCC, and could be regarded as a reference index for the diagnosis and treatment in LSCC.
出处
《现代肿瘤医学》
CAS
2013年第5期1025-1029,共5页
Journal of Modern Oncology
基金
国家自然科学基金课题(编号:81071933)
