摘要
目的监测并分析上海单中心住院腹泻儿童A组轮状病毒(RV)基因型别的变化特征,为RV性腹泻的防治提供基础数据和理论依据。方法收集2008年1月至2011年12月入住复旦大学附属儿科医院<5岁的腹泻患儿粪便标本,RV抗原阳性者345份,采用套式多重RT-PCR法进行RV的基因分型。结果①G基因型:2008至2010年以G3型为主,检出率分别为49.2%、44.6%和78.0%;G9型的流行呈上升趋势,成为2011年最主要的流行型别(51.1%);G1型在4年中均有检出(8%～20%);G2型少见,G4型未检出。G混合型中,以G3+G9型为主,其次为G3+G1型,还检出4例3种G基因型的混合。②P基因型:2008、2010和2011年均以P[8]型为主,检出率分别为55.6%、60.0%和68.1%,2009年以P混合基因型(43.2%)为主;P[4]型在4年均有流行;P[6]、P[9]型少见,仅在混合感染中检出P[10]型。P混合型中,以P[8]+P[4]型为主,其次为P[8]+P[10]型。③P[8]G3型是2008至2011年最主要的RV流行株(24.3%),其次为P[8]G9型及PmG3型。2011年P[8]G9型跃升为最主要的流行型别(40.5%)。结论与2001至2007年相比,2008至2011年上海地区A组RV基因型出现了新的流行特点,G1型及G9型的流行呈上升趋势,G3的流行有所下降,各种混合型别多见。对RV基因型保持系统性的连续监测对RV疫苗在上海的应用是必要的。
Objective This study aimed to analyze the dynamic characteristics of group A rotavirus genotypes. Methods A total of 345 rotavirus-positive stool specimens were collected from inpatients under 5 years old hospitalized with acute gastroenteritis in Children＇s Hospital of Fudan University from January 2008 to December 2011. Each fecal specimen was tested for rotavirus with a commercial enzyme immunoassay, and genotypes were characterized using multiplex nested reverse transcription polymerase chain reaction （RT-PCR）. Results G genotypes： The predominant G genotype was G3 which accounted for 49.2% , 44.6% and 78. 0% respectively in the year of 2008, 2009 and 2010. The prevalence of （;9 was on an upward trend from 2008 to 2011, and dominated as the most common type in 2011 （51.1%）. G1 was detected each year with a varying prevalence of 8% -20%. （;2 was rarely seen and G4 was not detected during the four years. Mixed G infections with G3 ＋ （;9 were the most prevailing G-mixed types, followed by G3 ＋ G1. Otherwise, mixed-G infections with three different G genotypes were detected in 4 cases. P genotypes ： P[ 8 ] genotype was the dominant strain with prevalence of 55.6% , 60.0% and 68.1% respectively in the year of 2008, 2010 and 2011, whereas mixed P genotype was dominant in 2009（43.2% ）. P[4] strains were prevalent in four years in a volatility way. P [6] and P[9] could also be found as minor types. Interestingly, P [ 10 ] genotype was seen only in combination with P[ 8 ]. The major mixed P infection was genotype of P [ 4] ＋ P [ 8 ], and P [ 8 ] ＋ P[ 10 ] was assigned as follows. A high prevalence of P [ 8 ] G3 genotype was determined to be the major rotavirus genotype combination （24.3%） in Shanghai from 2008 to 2011 and the other prevalent genotypes included P[ 8 ] G9 and P [ m ] G3. P [ 8 ] G9 strains jumped as the most predominant type in 2011 （ 40.5% ）. Conclusions Compared with the data from the year of 2001 -2007, this study shows new epidemiological characteristics of group A rotavirus genotypes during 2008 - 2011 in Shanghai, including ascending of G1 and （;9 proportions, reduction of G3 and diversification of mixed infections. Therefore, it is highly essential to conduct continuous and systematic monitoring for rotavirusstrains in anticipation of rotavirus vaccine introduction in Shanghai.
出处
《中国循证儿科杂志》
CSCD
2013年第2期98-104,共7页
Chinese Journal of Evidence Based Pediatrics
基金
新世纪优秀人才计划:NECT-09-0320
上海市公共卫生优秀学科带头人计划:GWDTR201221
