摘要
目的建立同时测定复方曲安奈德乳膏中醋酸曲安奈德和硝酸咪康唑含量的方法。方法采用RP-HPLC法。色谱柱为SunFire C18柱,流动相为甲醇-水(70∶30),检测波长为230nm,流速为1.0mL.min-1,柱温为30℃。结果醋酸曲安奈德和硝酸咪康唑质量浓度分别在0.008 4～0.050 4mg.mL-1(r=0.999 7)和0.049 8～0.298 8mg.mL-1(r=0.9998)范围内与峰面积积分值线性关系良好。平均回收率分别是98.3%,RSD为1.6%(n=9);98.6%,RSD为1.5%(n=9)。结论该方法准确、方便、快速,可用于复方曲安奈德乳膏中醋酸曲安奈德和硝酸咪康唑含量的测定。
Objective To establish a method for simultaneous determination of triamcinolone acetonide and miconazole nitrate in Com- pound Triamcinolone Acetonide Cream. Methods RP-HPLC was adopted. The determination was performed on a SunFire C18 col- umn with mobile phase consisted of methanol-water （70 ： 30） at 30 ℃. The flow rate was 1.0 mL · min-1 with detection wave- length at 230 nm. Results The linear ranges of triamcinolone acetonide and miconazole nitrate were 0. 008 4-0. 050 4 mg · mL-1 （r =0. 999 7） ,and 0. 049 8-0. 298 8 mg · mL-1 （r=0. 999 8） ,respectively with their average recoveries of 98.3% （RSD= 1.6% ,n= 9 ）, and 98.60% （ RSD~ 1.5 ~, n = 9 ）, respectively. Conclusion The method is accurate, simple, fast, and can be used for the deter- mination of triamcinolone acetonide and miconazole nitrate in Compound Triamcinolone Aeetonide Cream.
出处
《西北药学杂志》
CAS
2013年第3期252-254,共3页
Northwest Pharmaceutical Journal
