摘要
目的研究瑞舒伐他汀对大鼠颈动脉球囊损伤后内膜增生及线粒体融合素2表达的影响。方法选取雄性Wistar大鼠32只,随机分为假手术组、模型组、瑞舒伐他汀1组和瑞舒伐他汀2组,每组8只。瑞舒伐他汀1组于术前3天开始每日给予瑞舒伐他汀5 mg/kg灌胃,瑞舒伐他汀2组每日给予20 mg/kg灌胃,模型组以蒸馏水灌胃,随后制备颈动脉球囊损伤模型。建模后14天处死大鼠取颈动脉,HE染色行组织形态学观察,测量内膜/中膜面积比;免疫组织化学、免疫印迹法检测线粒体融合素2和增殖细胞核抗原的表达;依文思蓝染色观察血管内皮修复。结果与假手术组比较,模型组、瑞舒伐他汀1组和瑞舒伐他汀2组血管内膜增生,内膜/中膜面积比值显著增加(P<0.01),增殖细胞核抗原阳性细胞比值显著增加(P<0.01),线粒体融合素2表达显著降低(P<0.01);与模型组比较,瑞舒伐他汀1组、瑞舒伐他汀2组血管内膜/中膜面积比值显著降低(P<0.01),增殖细胞核抗原阳性细胞比值显著降低(P<0.01),线粒体融合素2表达显著增高(P<0.05),且瑞舒伐他汀2组较瑞舒伐他汀1组变化更显著(P<0.01)。依文思蓝染色显示,瑞舒伐他汀1组、瑞舒伐他汀2组再内皮化程度显著好于模型组(P<0.05)。结论瑞舒伐他汀可抑制大鼠颈动脉球囊损伤后内膜增生,同时促进内皮修复,其作用可能与上调线粒体融合素2表达有关。
Aim To investigate the effects of rosuvastatin on neointimal hyperplasia and mitofusin 2 （Mfn2） expression after carotid artery balloon injury in rats. Methods Thirty-two male Wistar rats were randomly divided into sham-operated group, model group, rosuvastatin group 1 and group 2. The rats in the two rosuvastatin groups were given rosuvastatin 5 mg/kg or 20 mg/kg daily, respectively. The rats in the model group and the two rosuvastatin groups were induced neointimal hyperplasia by carotid artery balloon injury. After the rats were sacrificed at day 14, HE staining was used to observe the change of vascular morphology. Immunohistochemistry staining and Western blot were performed to measure the expression of Mfn2 and proliferating cell nuclear antigen （PCNA）. Evans blue staining was used to measure the reendothlialized area. Results On the 14 th day after operation, compared with the model group, the neointimal area and intimal/media area ratio were significantly decreased in the two rosuvastatin groups （P〈0.01）. Mfn2 expression was up-regulated in the two rosuvastatin groups （P〈0.01）. Rosuvastatin did not inhibit the reendothlialized in the injured carotid arteries. Conclusions Rosuvastatin attenuates neointimal hyperplasia and restenosis in the rat carotid artery balloon injury model, which may be related to up-regulated expression of Mfn2.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2013年第5期424-428,共5页
Chinese Journal of Arteriosclerosis
