人参皂甙Rg1对大鼠关节软骨细胞凋亡的拮抗作用被引量：3

Ginsenoside Rg1 protects articular chondrocytes from apoptosis in rats

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摘要目的探讨人参皂甙Rg1对软骨细胞凋亡的影响。方法正常大鼠关节软骨细胞分为三组:A、B组用白细胞介素1β(IL-1β)10ng/ml作用24h,建立软骨细胞凋亡模型;B组事先加入10μg/ml的人参皂甙Rg1保护2h;C组作为空白对照。TUNEL法检测人参皂甙Rg1对软骨细胞凋亡的影响;Western blot检测人参皂甙Rg1对凋亡软骨细胞基质金属蛋白酶13(MMP-13)和基质金属蛋白酶组织抑制剂1(TIMP-1)蛋白表达的影响。结果 A、B和C组细胞凋亡率分别为(37.30±4.05)%、(10.30±1.08)%和(2.20±1.93)%,B组胞凋亡率低于A组(P<0.05)。与A组比较,B组软骨细胞TIMP-1的表达增加,MMP-13的表达降低(P<0.05)。结论人参皂甙Rg1具有拮抗大鼠关节软骨细胞凋亡的作用。 Objective To investigate the influence of Ginsenoside Rgl against interleukin-1 beta （IL-1β） - induced apoptosis in rat articular chondrocytes. Methods The articular chondrocytes were divided into three groups. The ehondrocytes in groups of A and B were exposed to IL-1β 10 ng/ml for 24 h to create apoptosis model. Ginsenoside Rgl 10 μg/ml was added at 2 h before IL-1β administration in group B. Group C was taken as blank control. The chondrocyte apoptosis was assessed by TUNEL staining and the expressions of MMP-13 and TIMP-i were detected by Western blot. Results The chondrocyte apoptosis rates in groups of A,B and C were （37. 30±4.05）%, （10. 30±1.08）% and （2.20± 1.93）%, respectively, which was lower in group B than that in group A（P〈0. 05）. Compared to group A, the expression of TIMP-1 was higher, but that of MMP-13 was lower, in group B （P〈0. 05）. Conclusion Ginsenoside Rgl can protect articular chondrocytes from apoptosis induced by IL-1β in rats.

作者吴东迎惠宇坚左强范卫民

机构地区南京医科大学第一附属医院骨科

出处《江苏医药》 CAS 北大核心 2013年第9期996-998,共3页 Jiangsu Medical Journal

基金国家自然科学基金(81000800) 江苏省自然科学基金(BK2011843)

关键词人参皂甙RG1 软骨细胞细胞凋亡 Ginsenoside Rgl Chondrocytes Apoptosis

分类号 R681 [医药卫生—骨科学]

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1Das SK,Farooqi A.Osteoarthritis[J].Best Pract Res ClinRheumatol,2008,22(4):657-675.
2Radad K,Gille G,Moldzio R,et al.Ginsenosides Rb1and Rg1effects on mesencephalic dopaminergic cells stressed withglutamate[J].Brain Res,2004,1021(1):41-53.
3Gong L,Li SL,Li H,et al.Ginsenoside Rg1protects primarycultured rat hippocampal neurons from cell apoptosis inducedby beta-amyloid protein[J].Pharm Biol,2011,49(5):501-507.
4Hong CE,Lyu SY.Anti-inflammatory and anti-oxidativeeffects of Korean red ginseng extract in human keratinocytes[J].Immune Netw,2011,11(1):42-49.
5Kang JH,Song KH,Woo JK,et al.Ginsenoside Rp1fromPanax ginseng exhibits anti-cancer activity by down-regulation of the IGF-1R/Akt pathway in breast cancer cells[J].Plant Foods Hum Nutr,2011,66(3):298-305.
6Sadowski T,Steinmeyer J.Effects of non-steroidal anti-inflammatory drugs and dexamethasone on the activity andexpression of matrix metalloproteinase-1,matrix metallo-proteinase-3and tissue inhibitor of metalloproteinases-1bybovine articular chondrocytes[J].Osteoarthritis Cartilage,2001,9(5):407-415.
7Attele AS,Wu JA,Yuan CS.Ginsengpharmacology:multipleconstituents and multiple actions[J].Biochem Pharmacol,1999,58(11):1685-1693.
8Wu J,Pan Z,Wang Z,et al.Ginsenoside Rg1protectionagainst beta-amyloid peptide-induced neuronal apoptosis viaestrogen receptor alpha and glucocorticoid receptor-dependentanti-protein nitration pathway[J].Neuropharmacology,2012,63(3):349-361.
9Du J,Cheng B,Zhu X,et al.Ginsenoside Rg1,a novelglucocorticoid receptor agonist of plant origin,maintainsglucocorticoid efficacy with reduced side effects[J].J Immunol,2011,187(2):942-950.
10Nevitt MC,Felson DT,Williams EN,et al.The effect ofestrogen plus progestin on knee symptoms and relateddisability in postmenopausal women:the heart and estrogen/progestin replacement study,a randomized,double-blind,placebo-controlled trial[J].Arthritis Rheum,2001,44(4):811-818.