摘要
采用质量分数为1.0%的明胶和1.78%的海藻酸钠作为固定化乳糖酶载体,比较了固定化乳糖酶随贮藏温度及适宜溶解pH值条件下的活力变化;同时,以牛乳水解率为指标,比较固定化乳糖酶水解乳中乳糖的效果表明,在-18℃及4℃下贮藏一个月,pH为4时,牛乳中的乳糖水解率仍然维持在75%以上。
This studydealt with the activity oflactaseimmobilized with 1.0% gelafinand 1.78% sodium alginate and then stored at different temperatures for one month.The optimal dissolved pH related to the release of the immobilized lactase was also discussed. Meanwhile, the immobilized lactase was applied to hydrolyzethe lactose ofinilk to evaluate the activity of the immobilized lactase stored at different temperatures. It was seen that the immobilized lactase stored at -18 ℃ or 4 ℃ kept the highest activity when the optimal pH was 4 during one-month storage. In this case, morethan 75% lactose from milk was hydrolyzed.
出处
《中国乳品工业》
CAS
北大核心
2013年第5期19-21,共3页
China Dairy Industry
基金
北京林业大学全日制专业学位研究生教育建设项目(090-0041)
北京林业大学理科生物学基地国家自然科学基金资助子项目(J1103516-ZD031)
北京林业大学2011年北京市大学生科学研究与创业行动计划项目(S111002213)
关键词
乳糖酶
固定化
温度
PH
lactase
immobilized carrier
storage temperatures
pH
