摘要
目的评价七氟醚后处理对大鼠肠缺血再灌注损伤的影响。方法成年雄性sD大鼠36只，体重200～220g，采用随机数字表法，将其分为4组（n=9）：假手术组（Sham组）、肠缺血再灌注组（I／R组）、缺血后处理组（Ipo组）和七氟醚后处理组（Sevo组）。I／R组、Ipo组Sevo组采用结扎肠系膜上动脉60min，再灌注120min的方法制备肠缺血再灌注模型。Ipo组于再灌注即刻恢复血流30s后再阻断30s，重复3次行后处理。Sevo组于再灌注即刻吸入1．15％七氟醚30min行后处理。再灌注120min时，处死大鼠，取小肠组织，采用Chui评分法行病理学损伤评分；采用TUNEL法计算凋亡细胞密度；采用比色法检测MDA含量和SOD活性；采用Westernblot法检测caspase-3蛋白表达。结果与Sham组相比，I／R组、Sevo组和Ipo组病理学损伤评分、凋亡细胞密度和MDA含量均升高，SOD活性降低，caspase-3蛋白表达上调（P〈0．05）；与I／R组相比，Sevo组和Ipo组病理学损伤评分、凋亡细胞密度和MDA含量均降低，SOD活性升高，caspase-3蛋白表达下调（P〈0．05）；Sevo组和Ipo组病理学损伤评分、凋亡细胞密度、MDA含量、SOD活性和caspase-3蛋白表达比较差异无统计学意义（P〉0．05）。结论七氟醚后处理可通过减轻脂质过氧化反应和减少细胞凋亡，减轻大鼠肠缺血再灌注损伤，其保护效应与缺血后处理相似。
Objective To investigate the effects of sevoflurane postconditioning on intestinal ischemia- reperfusion （I/R） injury in rats. Methods Thirty-six adult male Sprague-Dawley rats, weighing 200-220 g, were randomly divided into 4 groups （n = 9 each）： sham operation group （group Sham）, group I/R, ischemic post- conditioning group （group fpo） and sevoflurane postconditioning group （group Sevo）. Intestinal I/R was induced by clamping the superior mesenteric artery （SMA） for 60 min followed by 120 min of reperfusion in groups I/R, Ipo and Sevo. In group Ipo the animals were subjected to 3 cycles of 30 min reperfusion-30 rain ischemia starting from the beginning of reperfusion. The animals inhaled 1.15 % sevoflurane for 30 min starting from the beginning of reperfusion in group Sevo. The animals were sacrificed at 120 min of reperfusion and then the small intestines were removed for determination of malondialdehyde （MDA） content and superoxide dismutase （SOD） activity （by colori- metric method） and caspase-3 protein expression in intestinal tissues （by Western blot）. The density of apoptotic cells was calculated by TUNEL. Results Compared with group Sham, the intestinal injury score, density of apop- totic cells and MDA content were significantly increased, SOD activity was decreased, and caspase-3 protein ex- pression was up-regulated in groups I/R, lpo and Sevo （ P 〈 0.05）. Compared with group I/R, the intestinal inju- ry score, density of apoptotic cells and MDA content were significantly decreased, SOD activity was increased, andcaspase-3 protein expression was down-regulated in groups Ipo and Sevo （ P 〈 0.05 ）. There was no significant dif- ference in the intestinal injury score, density of apoptotic cells, SOD activity, MDA content and caspase-3 protein expression between Sevo and Ipo groups （ P 〉 0.05）. Conclusion Sevoflurane postconditioning can attenuate in- testinal I/R injury through reducing lipid peroxidation and cell apoptosis in rats, and the protective effect is similar to that of ischemic post-conditioning.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2013年第3期357-359,共3页
Chinese Journal of Anesthesiology
基金
基金项目：广东省自然科学基金（8151040701000062）
关键词
麻醉药
吸入
再灌注损伤
小肠
Anesthetics, inhalationg
Reperfusion injury
Intestine, small
