摘要
目的 探讨孕妇静脉血及其新生儿脐血硒水平及谷胱甘肽过氧化物酶 (GSH Px)活性与妊娠肝内胆汁淤积症 (ICP)的关系。方法 采用催化极谱法及 5 ,5 ' 二硫代双 (二硝基苯甲酸 )直接法检测健康未孕妇女 30例 (健康未孕组 )血硒水平及GSH Px活性 ;检测ICP孕妇 (ICP组 )和正常妊娠妇女 (正常妊娠组 )各 30例孕晚期及产后母血、脐血硒水平及母血、脐血GSH Px活性。结果 (1)ICP组产前、后母血硒水平分别为 (0 .0 389± 0 .0 0 90 )mg/L和 (0 .0 46 3± 0 .0 0 92 )mg/L ,GSH Px活性分别为 (5 9.31± 11.42 )活力单位 (U)和 (6 8.12± 11.46 )U ,正常妊娠组产前、后母血硒水平分别为 (0 .0 477± 0 .0 0 94)mg/L和 (0 .0 5 10± 0 .0 0 93)mg/L ,GSH Px活性分别为 (6 8.48± 10 .47)U和 (72 .6 7±9.83)U ,两组比较 ,差异均有显著性 (P <0 .0 0 5 )。 (2 )ICP组脐血硒水平为 (0 .0 387± 0 .0 0 93)mg/L ,GSH Px活性为 (5 7.6 6± 12 .2 5 )U ,正常妊娠组脐血硒水平为 (0 .0 46 1± 0 .0 0 89)mg/L ,GSH Px活性为(6 7.46± 11.93)U ,两组比较 ,差异均有极显著性 (P <0 .0 0 5 )。 (3)血硒水平与GSH Px活性呈正相关(P <0 .0 0 1)。结论 ICP患者血硒水平降低 ,导致GSH Px活性下降 ,自由基形成 。
Objective To investigate the importance of selenium concentration and glutathione peroxidase(GSH Px) activity in intrahepatic cholestasis of pregnancy (ICP). Methods We measured the selenium concentration by a catalytic polorographic method and GSH Px activity by a 5,5′ dithionbis (2 nitrobenzoic acid) direct method in maternal and cord blood in 30 pregnant women with and without this disease respectively during the last trimester of pregnancy and post partum and in 30 non pregnant women. Results Sults (1) The selenium concentration and GSH Px activity in maternal blood were found to be significantly lower in ICP than in women with normal pregnancies during the last trimester [(0.038 9±0.009 0) mg/L,(0.047 7±0.009 4) mg/L, P <0.001 ] [(59.31±11.42)U,(68.48±10.47)U, P <0.002] and post partum [(0.046 3±0.009 2)mg/L,(0.051 0±0.009 3)mg/L, P <0.05] [(68.12±11.46)U,(72.67±9.83)U, P <0.05]. (2)The selenium concentration and GSH Px activity in cord blood was significantly decreased in ICP than that of normal pregnances [(0.038 7±0.009 3) mg/L,(0.046 1±0.008 9) mg/L, P <0.005)] [(57.66±12.55)U,(67.46± 11.93)U , P <0.005)] (3) The activity of the selenoenzyme GSH Px had a significant positive correlation with selenium concentration ( P <0.001). Conclusion The blood selenium deficiency and reduced GSH Px activity may lead to the formation of free radicals, which could damage the structure and function of hepatocytes and result in ICP.
出处
《中华妇产科杂志》
CSCD
北大核心
2000年第9期520-522,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
胆汁郁积
肝内
妊娠
硒
谷胱甘肽过氧化酶
Cholestasis
intrahepatic
Pregnancy
Selenium
Glutathione peroxidase
