摘要
为探讨种植受者血管内皮细胞在抗异种移植超急性排斥反应方面的意义,选用豚鼠→大鼠移植模型，先分离、培养大鼠动脉内皮细胞，然后将其种植于去内皮的豚鼠动脉内壁，并以免疫组化技术检测了种植大鼠内皮细胞的豚鼠动脉与大鼠血清反应的情况。结果显示：大鼠血清与正常的豚鼠动脉一起培养后，前者所含IgM、C3沿后者内皮细胞表面沉积；种植大鼠内皮细胞的豚鼠动脉与大鼠血清反应后免疫荧光反应呈阴性，即无IgM和C3。以上结果表明，血管内皮细胞可在异种血管壁上种植，如将受者血管内皮细胞预先种植于供者的血管内壁，可能避免超急性排斥反应的发生。
The potential solution to palliate the critical shorta ge of suitable donor organs for transplantation may be xenogenic transplantation However, hyperacute rejection (HAR) after xenotransplantation is the main prob lem in this process. Since the vascular endothelial cell of donor organ is the p rimary target cell in rejection, the replacement of endothelium in donor organs with endothelial cell from recipients themselves may be beneficial to the prevention of HAR. Dis cordant xenotransplantation model (guinea pig-to-rat) was adopted in this stud y. Firstly, endothelial cell from rat abdominal aorta was separated and cultured. Secondly, the guinea pig abdominal aorta of which the endothelium had been remo ved was cultured with the suspension containing rat endothelial cells (4*106/ml). The viability of cultured vessel was assessed using light m icroscopy and transmission electron microscopy. The guinea pig vessel reseeded wit h rat endothelial cell was then examined by immuno-fluorescence staining assay to find out whether IgM and C3 in rat serum were bound to it after preincubation with rat serum. It was found that rat endothelial cells grew into the monolayer e ndothelium on the inner surface of guinea pig vessel with previous endothelial l oss. IgM and C3 in rat serum did not deposit along the new endothelium of guinea pig vessel in the treated group as shown by immuno-fluorescence microscopy, wh ereas possive results were observed in the untreated normal guinea pig aorta. The se findings indicate that donor vessel reseeded with endothelial cell from recip ients undergoes less severe rejection and this technique may be very useful for the attenuation of HAR.
出处
《华西医科大学学报》
CSCD
2000年第3期344-346,352,共4页
Journal of West China University of Medical Sciences
基金
国家自然科学基金!(批准号 39500151)
