摘要
筛选具有抑制植物病原菌活性的放线菌是农用抗生素开发的基础。通过平板对峙法筛选出8株拮抗放线菌菌株,采用生长速率法测定获得一株对植物病原菌有较好抑菌作用的菌株K2,并采用抑制菌丝生长速率法对其抑菌谱进行研究,以及进行菌种鉴定。生长速率法试验表明,放线菌菌株K2发酵液对10种供试植物病原菌菌丝生长均有不同程度的抑制作用,其中以对尖孢镰刀菌(Fusarium oxysporum)抑制效果最好,抑菌率可达57.38%。根据形态学特征、生理生化特征和基于16SrDNA序列比对,鉴定菌株K2属于链霉菌属(Streptomyces)的一个菌株。放线菌K2的代谢产物具有较广的抑菌谱,对植物病原菌的防治有很大的研究开发潜力。
Screening antifungal actinomycetes were the foundation of agricultural antibiotics exploitation. The antifungal activity of 8 strains actinomycetes were studied by fiat confrontation, one strain named K2 was got by mycelim growth rate, its antimicrobial spectrum was studied and strains identification with mycelim growth rate. The result of mycelium growth rate showed that K2 had a broad spectrum inhibitory activity to all the tested 10 plant pathogenic fungi. Among these, activity against Fusarium oxysporum was the highest with inhibition rate of 57.38%. The results based on morphological characteristics, physiological and biochemical characterization and 16S rDNA sequence analysis of strain K2 showed that it was possibly Streptomyces. The K2 strain displayed a broad spectrum inhibitory activity, which had great potential for controlling plant pathogens.
出处
《中国农学通报》
CSCD
2013年第15期73-77,共5页
Chinese Agricultural Science Bulletin
基金
甘肃省植物源生物农药工程技术研究中心项目(1106NTGA013)
关键词
放线菌
筛选
发酵液
抑菌活性
鉴定
actinomycetes
screen
fermentation
antifungal activity
identification
