摘要
采用A(lNO3)3络合分光光度法测定芹菜不同部位70%乙醇提取物中总黄酮含量;以VC为阳性对照,通过测定对羟自由基(.OH)、超氧阴离子自由基(O2-.)、亚硝酸盐(NO2-)的清除作用及总还原力,评价不同部位提取物的抗氧化性能。结果表明:芹菜不同部位总黄酮含量差异较大,叶中总黄酮含量最高(40.71 mg/g),茎次之(10.82 mg/g),根最低(7.53 mg/g);在不同的抗氧化体系中,芹菜根、茎、叶提取物均表现出较强的抗氧化活性,且具有剂量效应关系,同等条件下,芹菜叶提取物对羟自由基(.OH)清除作用、超氧阴离子自由基(O2-.)清除作用和还原能力最强,芹菜叶提取物对亚硝酸盐(NO2-)的清除作用最强,3种提取物对(.OH)清除作用均强于VC。
In this paper, the total flavonoids contents of 70 % ethanol extracts from three different tissues of celery were determined by the A1 （NO3）3 complexation speetrophotometric method. Using Vc as a positive control, antioxidant activity of the extracts were tested and compared by hydroxyl radicals （. OH） scavenging activity, superoxide anion radical （O2-·） scavenging activity, nitrite radical （NO2-） scavenging activity and reducing power. Results indicated that the total flavonoids contents in different tissues of celery had significant difference, the total flavonoids content in leaves was highest （40.71 mg/g）, followed by that in stems （10.82 mg/g）, and the third was in roots （7.53 mg/g）. All the three extracts exhibited strong antioxidant activity in a concentration-dependent fashion in the various antioxidant system. The extracts from celery leaves had the strongest ability to scavenge · OH and O2-· free radicals and reducing power, and the extracts from celery stems had the strongest ability to scavenge NO2-, all the three extracts from celery leaves, stems and roots presented higher · OH scavenging activity than Vc.
出处
《食品研究与开发》
CAS
北大核心
2013年第7期12-15,共4页
Food Research and Development
基金
陕西理工学院校级重点资助科研项目(SLGKY11-09)
关键词
芹菜
总黄酮
抗氧化活性
celery
total flavonoids
antioxidation activity
