摘要
采用索氏提取和超声波提取两种方法进行尾巨桉(DH32-29)叶片脂肪酸的提取,并利用GC-MS技术分析脂肪酸成分。结果表明:两种提取法均检出尾巨桉叶片含6种脂肪酸,其中4种饱和脂肪酸,分别为十四酸、棕榈酸、硬脂酸、花生酸;2种多不饱和脂肪酸,为亚油酸和α-亚麻酸;各成分含量均以α-亚麻酸、棕榈酸、亚油酸为主;索氏法提取粗脂肪酸的提取率(7.64%)高于超声波法(6.12%);索氏法提取的多不饱和脂肪酸成分占提取粗脂总量的21.30%,主要为亚油酸(20.67%)和α-亚麻酸(0.63%)。
The fatty acids in Eucalyptus urophylla ~ E. grandis （ DH32_29 ） leaves were extracted by two methods （ Soxhlet ex- traction and ultrasonic extraction）, and its compositions were analyzed by GC-MS. The results showed that there were six kinds of fatty acids, four of them were saturated fatty acids, tetradecanoate, hexadecanoic acid, octadecanoic acid and eico- sanoic acid, two of them were polyunsaturated fatty acids, （ Z, Z, Z） -9,12,15 -octadecatrienoic acid and （ Z, Z） -9,12- octadecadienoic acid, and the main fatty acids were （Z, Z, Z） -9,12,15 -octadecatrienoic acid, hexadecanoic acid, and （ Z, Z） -9,12-octadecadienoic acid. Extraction rate of Soxhlet method （7.64%） was higher than that of ultrasonic extrac- tion （6.12%） significantly. The polyunsaturated fatty acids was accounting for 21.30% of total extraction by Soxhlet ex- traction, which were mainly （ Z, Z） -9,12-octadecadienoic acid （ 20.67 % ） and （ Z, Z, Z ） -9,12,15 -octadecatrienoic acid （0.63%）.
出处
《林业科技开发》
北大核心
2013年第3期86-89,共4页
China Forestry Science and Technology
基金
广东省自然科学基金项目(编号:9145624536-4000003)
湛江市财政资金科技专项竞争性分配项目(编号:20102030244)
林业重大公益性行业科研专项(编号:201104003)
