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细胞凋亡机制与主要检测方法研究进展 被引量:3

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摘要 细胞凋亡是一种由基因调控的细胞自主性死亡过程,在维持动物机体内环境平衡中扮演着极其重要的角色,是目前生命科学和医学领域各专业研究的一个热点。本文概述了与细胞凋亡的作用机制相关的基因:半胱氨酸基天冬氨酸-特异性蛋白酶(cysteinyl aspartate specific protease,Caspase)、Bcl-2基因家族、P53蛋白等,以及细胞凋亡的检测方法,比如形态学观察法、DNA片段化检测、流式细胞仪分析(flow cytometry assay,FCA)和凋亡相关因子Cas-pase活性的检测。为进一步了解细胞凋亡在疾病中发挥的作用以及对细胞凋亡作出快速、准确的检测奠定基础。
出处 《畜牧兽医科技信息》 2013年第5期6-8,共3页 Chinese Journal of Animal Husbandry and Veterinary Medicine
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