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2011年甘肃地区急性呼吸道感染儿童患者中鼻病毒感染规律的研究 被引量:11

Characterization of Human Rhinovirus in Children with Acute Respiratory Infections in Gansu Province during 2011
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摘要 探讨人鼻病毒(Human rhinovirus,HRV)在甘肃地区急性呼吸道感染儿童患者中的流行特征。2011年1月至12月,从甘肃地区急性呼吸道感染儿童患者中采集咽拭子标本286份,用多重PCR方法对常见呼吸道病毒性病原体进行筛查。多重PCR结果中HRV阳性的标本再用nested-PCR分别扩增HRV的VP1和VP4/VP2结合区基因片段,阳性结果进行序列测定和基因分析。286份咽拭子标本中,27份标本多重PCR检测结果显示为HRV阳性,检出率为9.44%。本研究中,HRV感染病例的平均年龄为3岁,其中,1岁以内儿童感染比例最高,占总阳性数的44.4%(12/27);在27例HRV阳性病例中,男性占16例(16/185,8.64%),女性占11例(11/101,10.89%),两者无显著性差异;HRV感染的高峰出现在5月份(6/27,22.2%);从HRV感染的疾病构成看,普通感冒的比例最高,占12.24%(12/98);其次为肺炎,占8.50%(13/153);剩余2病例均为支气管炎。基因序列特征分析发现:A组HRV是甘肃地区2011年的优势流行基因型,占阳性病例的84.62%(22/26),其次为C组HRV,占11.54%(3/26),B组HRV仅发现1例。HRV感染在2011年甘肃地区全年均可发生,主要感染以1岁以内儿童。A组HRV是该地区2011年急性呼吸道感染儿童患者中的主要流行基因型,并存在多个基因型和多个HRV血清型共同流行的特点。 To study the epidemic characteristics of human rhinovirus (HRV) in children with acute respira- tory infections in Gansu Province. 286 throat swabs were collected from children with acute respiratory in- fections in Gansu Province during 2011. Multiplex reverse transcription-PCR (multiplex RT-PCR) assay was used to screen those specimens for detection of common respiratory tract pathogens. For HRV-posi- tive samples, nested reverse transcription polymerase chain reaction (nested RT-PCR) was performed to amplify VP1 and VP4/VP2 gene fragments of HRV. The VP4/VP2 and VP1 regions of HRV-positive samples were sequenced and performed genotype analysis. Of 286 specimens fested, 27 were positive for HRV by multiplex RT-PCR and nested RT-PCR, of which 16 children were made(16/185), 8.64%) and 11 female (11/101,10.89G). The positive rate was 9. 44% (27/286). The mean age of HRV-positive children was 3 years in this study, children less than one year old had the highest proportion 44.4%(12/ 27,44.4%). The highest HRV positive rate fell on May, 2011 (6/27,22.2%). Common cold accounted for the highest proportion, 12.24%(12/98) followed by pneumonia, 8.50%(13/153). The remaining 2 cases were bronchitis. Sequence analysis showed HRV A was the predominant genotype in Gansu Province in 2011, accounting for 84. 62%(22/26) of positive cases, followed by HRV C(11.54%, 3/26) and only one HRVBwas detected (3.85G, 1/26). HRVcould be detected throughout the year in Gansu Province and primarily infected children under one year old. The group A was the epidemic genotype of HRV and move than one genotype existed in Gansu Province during 2011.
出处 《病毒学报》 CAS CSCD 北大核心 2013年第3期273-279,共7页 Chinese Journal of Virology
基金 (艾滋病和病毒性肝炎等重大传染病防治科技重大专项)病毒性传染病病原谱流行规律及变异研究(2013ZX10004202) (艾滋病和病毒性肝炎等重大传染病防治科技重大专项)甘肃及周边省区传染病病原谱流行规律研究(2012ZX10004-208) (艾滋病和病毒性肝炎等重大传染病防治科技重大专项)重大传染病应急处置检测技术平台(2011ZX10004-001)
关键词 人鼻病毒 急性呼吸道感染 多重PCR 基因型 Human rhinovirus (HRV) : Acute resDiratorv in{ections: MuhiDlex RT-PCR GenotvDe
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同被引文献87

  • 1邹晓华,王春英,蒋颖超.甲强龙与地塞米松治疗重症哮喘的疗效对比分析[J].中国误诊学杂志,2005,5(6):1044-1045. 被引量:11
  • 2阎锡新,张海林.糖皮质激素对急性呼吸窘迫综合征治疗价值与机制的研究进展[J].中华内科杂志,2006,45(3):256-258. 被引量:7
  • 3社区获得性肺炎诊断和治疗指南[J].中华结核和呼吸杂志,2006,29(10):651-655. 被引量:3056
  • 4李军,朱启镕,俞蕙,顾新焕.上海地区儿童急性下呼吸道常见病毒及鼻病毒感染的临床研究[J].临床儿科杂志,2007,25(6):457-461. 被引量:37
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  • 6Simons E,Schroth MK,Gem JE.Analysis of tracheal secretions for rhinovirus during natural colds[J] .Pediatr Allergy Immunol, 2005,16(3) :276-278.
  • 7Lau SK,Yip CC,Lin AW,et al.Clinical and molecular epidemiology of human rhinovirus C in children and adults in Hong Kong reveals a possible distinct human rhinovirus C subgroup[J].J Infect Dis,2009, 200(7) : 1096-1103.
  • 8Peiris JS,Tang WH,Chan KH,et al.Children with respiratory disease associated with metapneumovirus in Hong Kong [J]. Emerg Infect Dis, 2003,9 ( 6 ) : 628-633.
  • 9Coiras MT,Porez-Brena P,Garcia ML,et al.Simultaneous detection of influenza A,B,and C viruses, respiratory syncytial virus, and adenoviruses in clinical samples by multiplex reverse transcription nested-PCR assay [J ] .J Med Virol, 2003,69 ( 1 ) : 132-144.
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