摘要
背景:骨碎补能在促进骨生长且取得了良好的临床疗效,其主要成分柚皮甙能否诱导骨髓间充质干细胞向成骨方向分化?目的:用柚皮甙诱导兔骨髓间充质干细胞向成骨方向分化。并观察柚皮甙诱导兔骨髓间充质干细胞向成骨方向分化的能力。方法:用贴壁筛选法对兔骨髓间充质干细胞进行分离培养。对生长良好的第3代骨髓间充质干细胞分别用50μg/L的柚皮甙和经典成骨诱导剂向成骨方向进行诱导,分别进行成骨鉴定。结果与结论:经典成骨诱导液、柚皮甙诱导液都能使骨髓间充质干细胞向成骨方向分化,基质分泌增多,形成钙结节。用酶联免疫检测仪检测柚皮甙诱导组和经典成骨诱导组细胞吸光度值未见明显的差异。同时检测柚皮甙诱导液和L-DMEN细胞培养液的吸光度值发现两者差异无显著性意义(P>0.05)。证实柚皮甙可成功诱导骨髓间充质干细胞向成骨方向分化,无明显毒性作用。
BACKGROUND： Drynaria can promote bone growth and has made good clinical curative effect. Can the main ingredients of Naringin induce bone marrow measenchymal stem cells to differentiation into osteoblasts？ OBJECTIVE： Through the use of Naringin to induce osteogenic differentiation of rabbit bone marrow mesenchymal stem cells, to observe the osteogenic ability of Naringin-induced rabbit bone marrow mesenchymal stem cells. METHODS： Adherence screening method was used to separate and culture the rabbit bone marrow mesenchymal stem cells. The well-grown third generation of bone marrow measenchymal stem cells wereinduced with 50 ug/L Naringin and classic osteogenic inducer respectively to differentiate into the osteoblasts and the osteogenesis identification was performed. RESULTS AND CONCLUTION： Both the classic osteogenic inducer and Naringin induced liquid could induce the bone marrow mesenchymal stem cells to differentiation into osteoblasts with increasing matrix secretion and calcium nodules formation. The enzyme-linked immunosorbent assay showed that there was no significant difference in absorbance value of the cells between the Naringin induction group and the classic osteogenic inducer group. There was no significant difference in absorbance value of the cells induced with Naringin induced liquid and L-DMEN culture medium （P 〉 0.05）. The results indicate that Naringin can induce the bone marrow mesenchymal stem cells to differentiate into osteoblasts without obvious toxic effect.
出处
《中国组织工程研究》
CAS
CSCD
2013年第14期2603-2608,共6页
Chinese Journal of Tissue Engineering Research
基金
广西科技厅资助项目(桂科攻0816004-15)
广西卫生厅资助项目(重200863)~~
关键词
干细胞
干细胞培养与分化
柚皮甙
骨髓间充质干细胞
成骨细胞
细胞分化
成骨诱导
细胞培养
碱性磷酸酶
Ⅰ型胶原
省级基金
干细胞图片文章
stem cells
stem cell culture and differentiation
Naringin
bone marrow mesenchymal stem cells
osteoblasts
cell differentiation
osteogenic induction
cell culture
alkaline phosphatase
collagen type I
provincial grants-supported paper
stem cell photographs-containing paper
