摘要
背景:细菌感染是影响伤口愈合的主要因素之一,伤口渗出液里含有的大量炎症因子、蛋白酶和自由基都会减缓伤口的愈合速度。新型复合生物抗菌敷料的研发对治疗外科感染伤口有重要的意义,是创伤敷料发展的必然趋势。目的:观察添加纳米银的海藻酸钙敷料的抗菌活性、吸湿能力及细胞毒性。方法:将纳米银材料添加到海藻酸钙中制备新型复合生物抗菌敷料,并通过使用平板计数法、MTT法、电子显微镜观察法观察敷料的抗菌活性、吸湿能力及细胞毒性,再与银离子海藻酸钙敷料和海藻酸钙敷料进行对比,以期显示出新型复合生物抗菌敷料的具有强抗菌性及低细胞毒性的优势。结果与结论:与银离子海藻酸钙敷料和海藻酸钙敷料相比,添加纳米银的新型复合生物抗菌敷料对金黄色葡萄球菌、铜绿假单胞菌均有更强的抑菌作用(P<0.01),细胞毒性较低(P<0.01);3种敷料的吸湿能力差异无显著性意义。证实此添加纳米银的海藻酸钙敷料的具有强抗菌性及低细胞毒性。
BACKGROUND：Bacterial infection is one of the main factors affecting wound healing. Wound exudate containing large amount of inflammatory cytokines, proteases and free radicals wil delay wound healing. Research and development of new compound biological antimicrobial dressings have important implications for treating surgery wound infection, which is the inevitable trend of the development of wound dressings. OBJECTIVE：To observe the antibacterial activity, moisture absorption capacity and cytotoxicity of calcium alginate dressing containing nanocrystal ine silver. METHODS：Silver nanoparticles were added into calcium alginate to prepare new compound biological antimicrobial dressings. The antibacterial activity, moisture absorption capacity and cytotoxicity of calcium alginate dressing containing nanocrystal ine silver were detected using the plate count method, 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide method, electron microscope experiment, compared with silver ion calcium alginate dressing and calcium alginate dressing, in the hope of showing the strong antimicrobial activity and low cytotoxicity of new compound biological antimicrobial dressings. RESULTS AND CONCLUSION：The new compound biological nano-silver antimicrobial dressing, compared with the silver ion calcium alginate dressing and calcium alginate dressing, had stronger bacteriostasis to Staphylococcus aureus and Pseudomonas aeruginosa （P〈0.01）, and exhibited lower cytotoxicity （P〈0.01）. There occurred no significant differences in moisture absorption capacity among the three groups （P〉0.05）. It is proved that the new compound biological nano-silver antimicrobial dressing has stronger antibacterial activity and lower cytotoxicity.
出处
《中国组织工程研究》
CAS
CSCD
2013年第16期2905-2912,共8页
Chinese Journal of Tissue Engineering Research
关键词
生物材料
生物材料与药物控释
新型生物敷料
纳米银
海藻酸纤维
抗菌
吸湿
细胞毒性
扫描电子显微镜
biomaterials biomaterials and control ed drug release novel biological dressings nano-silver alginate fiber antibacterial moisture absorption cytotoxicity scanning electron microscope
