摘要
目的溴甲酚紫法体外测试黄连素的抗阴道毛滴虫活性.方法设计实验组、已知疗效对照组和阴性对照组,溴甲酚紫比色法体外测定药物作用于阴道毛滴虫后不同时间、不同药物浓度的培养基OD值,经线性回归分析,绘制IC50曲线图,进行药效学比较分析.结果药物作用早期(2 h),黄连素IC50值高于甲硝唑;4～6 h 2种药物的IC50值较接近,12 h时,甲硝唑的IC50值为11.64μg/mL,黄连素的IC50值为10.58μg/mL,24 h,黄连素、甲硝唑的IC50值继续下降,在9.53～9.61μg/mL之间.48 h,两药的IC50值在5.38～6.03μg/mL之间,黄连素IC50值稍高.结论黄连素体外抗阴道毛滴虫的活性与甲硝唑相当,作为治疗阴道毛滴虫感染或治疗耐甲硝唑虫株的替代药物,具有很好的开发利用前景.
Objective To test in vitro the anti-trichomonas vaginalis activity of berberine by bromocresol purple assay. Methods The Trichmonas vaginals in experimental group, known-efficacy group and negative group, were tested by applying the bromocresol purple colorimetric spectroscopic assay. Before and after the treatment, the OD values of culture medium were measured at different times to make the linear regression analysis and draw IC50 curves. Results Compared at the earlier incubation period （2 h） , the IC50 value of berberine was higher than that of metronidazole. During the 4 h and 6 h, the IC50 values of two medicaments were similar. At 12 h, the IC50value of metronidazole was 11.64 μg/mL and that of berberine was 10.58 μg/mL. At 24 h, the the ICso values of two medicaments kept decreasing, were between 9.53 and 9.61 μg/mL. At 48 h, the IC50 values of two medicaments were between 5.38 and 6.03 μg/mL while the IC50 value of berberine was higher. Conclusion The berberine has similar anti-trichomonas vaginalis activity with metronidazole, and can substitute metronidazole in treating the trichomonas vaginalis infection and the metronidazole-resistant strains infection, so it has good application prospects.
出处
《昆明医科大学学报》
CAS
2013年第4期13-15,27,共4页
Journal of Kunming Medical University
基金
云南省自然科学基金资助项目(2010ZC111)
关键词
阴道毛滴虫
抗滴虫药物
体外培养
黄连素
Trichmonas vaginals
Anti-triehmonas drugs
Culture in vitro
Berbeine
