摘要
酵母真核表达系统具有生长迅速、安全性好、发酵密度高等优点,对于外源蛋白的大规模工业化生产具有重要的意义。由于外源蛋白的产量常常受到一些内源性因素的影响,因此针对发酵工艺、载体系统及宿主菌株等方面的工程化改造已成为研究的重点,其中通过遗传修饰对菌株的工程化改造可以有效克服酵母分泌途径中的不足。目前的菌株改造策略主要针对外源蛋白酵母分泌系统、蛋白酶降解及糖基化修饰等方面,其中酵母分泌系统涉及许多互相影响制约的因素,对其进行的遗传修饰也最为复杂困难。本文针对最新的酵母分泌系统工程化改造策略及其优点进行综述。
Yeast is a type of widely used eukaryotic microorganism easy to do genetic manipulation and fermentation, which has capability to secrete and modify foreign proteins. Its advantages of rapid growth, high safety and high density fermentation are significant for foreign proteins industrial productions. In some cases, however, the production of foreign proteins in yeast system is often limited by some endogenous factors. Recently, many researches have focused on engineering of fermentation process, vector systems and host strains, which effectively overcome the disadvantages in the yeast secretion pathway. At present, engineering strategies for yeast strains focus on yeast secretion system, protease degradation and glycosylation. Yeast secretion system involves many cross-reacting factors making its genetic modification more complicated. The recent strategies for yeast secretion system and their advantages are reviewed in this article.
出处
《生物技术进展》
2013年第3期185-189,共5页
Current Biotechnology
基金
国家973计划项目"生物活性分子结构和功能的关系研究"(2012CB724500)资助
关键词
酵母
分泌系统
蛋白质折叠
蛋白质运输
yeast
secretion system
protein folding
protein trafficking
