摘要
为探讨川白芷不同器官内源激素的提取纯化和分析测定,在前人研究的基础上,建立了一种从川白芷不同器官组织中快速提取纯化并同步测定4种植物内源激素(玉米素、赤霉素、3-吲哚乙酸和脱落酸)的高效液相色谱分析方法。样品经80%甲醇提取后低温浓缩,最后用甲醇定容。采用Tigerkin C18柱(150mm×4.6mm,5μm)以甲醇—0.6%乙酸(50:50)为流动相进行高效液相色谱分离,PDA光电二极管阵列检测器检测,采用峰面积外标法(ESTD)进行定量。结果表明,该方法具有简单快捷,准确灵敏等优点,适合对川白芷内源激素进行快速同步测定。
A simple and rapid method using High Pressure Liquid Chromatography （HPLC） for the simultaneous determination of four representative endogenous hormones including Gibberellic acid （ GA3 ）, Zeatin （ZT）, Indoleacetic acid （IAA） and Abscisic acid （ABA） in Angelica dahurica, was described. Endogenous hormones were extracted using ethanol solution （80% , V/V） under low-temperature condition. Then the extracting sample was separated by HPLC with a C18 ODS column （150 mm× 4.6mm, 5μm） using methanol - 0.6% acetic acid （50：50, v/v） as mobile phase. The signal was detected by Photodiode Array （PDA） , and the external standard method （ESTD） was employed for quantitative analysis. The experimental results demonstrated this method was an accurate and high-separation- efficiency technique to analyze trace amounts of the four endogenous hormones in various organs of Angelica dahurica.
出处
《核农学报》
CAS
CSCD
北大核心
2013年第5期653-657,共5页
Journal of Nuclear Agricultural Sciences
基金
四川省育种攻关项目(2011NZ0098-12-09)
