摘要
通过在一定地理间隔设立采样点,采集了8个地区的野生黄檗韧皮部样品,采用高效液相色谱方法测定黄檗(Phellodendron amurense Rupr.)韧皮部的药根碱、掌叶防己碱和小檗碱质量分数,分析了1月份、3—7月份、5 a部分气象数据平均值与黄檗中小檗碱、药根碱、掌叶防己碱质量分数的关系。结果表明:药根碱质量分数与5 a平均极低温度具有极显著的正相关性,与5 a平均相对湿度存在负相关趋势;掌叶防己碱质量分数与3—7月平均降水、月温较差、5 a平均相对湿度成负相关趋势;小檗碱质量分数与1月份均温、5 a平均极低温度具有极显著的正相关性。主成分分析得出,温度是影响黄檗生长的首要因子,其次为湿度、降水、光照、地理因子等。逐步回归分析得出:较低的5 a平均相对湿度有利于黄檗树皮中药根碱的积累;较高5 a平均极高温度,较低5 a平均相对湿度的低经、纬度区域有利于掌叶防已碱的积累;较高5 a平均极低温的低经、纬度区域有利于黄檗树皮中小檗碱的积累。海拨在相关性分析和多元线性逐步回归分析中与3种生物碱的含量没显著的相关性,
Phloem of weild Phellodendron amurense Rupr. of eight different areas was selected from different geography distribution sample sets. An experiment was conducted to study the sample contents of berberine, jatrorrhizine and palmatine by using High Performance Liquid Chromatography and analyze the relationship between the climate factors from the averages of January, March to July and five years, and the contents of berberine, jatrorrhizine and palmatine. There is a significant positive relationship between jatrorrhizine content and average lower temperature of five years. There is a negative relation- ship between the jatrorrhizine content and relative humidity of five years. There is negative relationship between the palmatine content and average precipitation from March to July, temperature differences among months and average relative humidity of five years. There is significantly positive relationship between the berberine content and the average temperature of January, and the lowest temperature of five years. The relations between alkaloid contents and climate factors were stud- ied by correlation analysis. The principal effects of ecological factors on Amur corktree growth are temperature, humidity, precipitation and sunlight. The relations between alkaloid contents and climate factors were studied by muhiple regression analysis. The most suitable climate conditions for the accumulation of jatrorrhizine are higher mean temperature in January and lower annual relative humidity. The most suitable climate conditions for the accumulation of palmatine are higher annual mean temperature, higher annual extreme high temperature, lower annual relative humidity, lower latitude and lower longitude. The most suitable climate conditions for the accumulation of berberine are higher annual extreme low temperature, lower latitude and lower longitude. There is no significant correlation between the alkaloid contents and altitude by multiple regression analysis and correlation analysis.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2013年第6期14-18,共5页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(30271045)
关键词
气候因子
黄檗
小檗碱
药根碱
掌叶防己碱
Climate factor
Corktree （Phellodendron amurense）
Berberine
Jatrorrhizine
Palmatine
