摘要
以黄瓜枯萎病菌(Fusarium oxysporum)、稻瘟病菌(Magnaporthe grisea)、柑橘青霉菌(Penicillium digitatum)、番茄灰霉病菌(Botrytis cinerea)、梨黑星病菌(Venturia nashicola)5种植物病原真菌为供试菌种,采用牛津杯法,研究了野葛花乙醇提取物的抑菌活性及其对植物病原真菌的抑菌效果。结果表明:野葛花的乙醇提取物对这5种植物病原真菌均有不同程度的抑制作用;随着提取物浓度的增大,其对植物病原真菌的抑菌效果逐渐增强,当达到某一值后,抑制效果又随其浓度的增加逐渐减弱。另外,黄瓜枯萎病菌(Fusarium oxysporum)、稻瘟病菌(Magnaporthe grisea)、柑橘青霉菌(Penicillium digitatum)、番茄灰霉病菌(Botrytis cinerea)、梨黑星病菌(Venturia nashicola)的最适野葛花乙醇提取物抑菌质量浓度分别为0.1250、0.2500、0.2500、0.2500、0.5000g/mL,其中番茄灰霉病菌的最低抑菌质量浓度应大于0.0625g/mL。因此,野葛花的乙醇提取物在抑制植物真菌性病害方面具有广阔的开发利用前景,可作为新型植物源生物农药的良好剂型。
Based on the ethanol extractions of Pueraria lobata（Willd.） Ohwi flowers,the inhibition effect and the antibacterial activity on Fusarium oxysporum,Magnaporthe grisea,Penicillium digitatum,Botrytis cinerea and Venturia nashicola 5 kinds of plant pathogenic fungus were tested with Oxford cup method in different concentration extraction.The results showed that there were different degrees of inhibition effect of the ethanol extractions of Pueraria lobata（Willd.） Ohwi flowers on the 5 kinds of fungus.With the extractions concentration increasing,the inhibition effects were strengthened,and then weakened when up to a certain value.In addition,the optimal extraction concentrations of Fusarium oxysporum,Magnaporthe grisea,Penicillium digitatum,Botrytis cinerea and Venturia nashicola were 0.1250,0.2500,0.2500,0.2500,and 0.5000 g/mL;and the minimum concentration to Botrytis cinerea should be greater than 0.0625 g/mL.Therefore,there were broad development and utilization prospect of the ethanol extractions of Pueraria lobata（Willd.） Ohwi flowers in inhibiting plant fungal disease,and could be used as a new type plant source bio-pesticide form.
出处
《北方园艺》
CAS
北大核心
2013年第11期116-118,共3页
Northern Horticulture
基金
云南省高校科技创新团队资助项目(500947)
云南省风景园林学省级重点学科资助项目(23002802)
国家林业局西南风景园林工程技术研究中心资助项目
关键词
野葛花
乙醇提取物
植物病原真菌
抑菌作用
Pueraria lobata（Willd.） Ohwi
ethanol extractions
plant pathogenic fungus
inhibition effect
