摘要
采用平板分离法从健康的"红富士"苹果果皮筛选到3株拮抗细菌,通过活体试验和离体试验研究了拮抗细菌对青霉的抑制作用。结果表明:3株细菌都具抑菌活性,其中菌株BF-2的防效可达87.6%,而对峙试验中菌株BF-3的抑制率达33.3%。利用牛肉膏蛋白胨液体培养基发酵拮抗细菌,发现发酵液具抑菌活性,其中BF-3发酵液的抑菌圈为27.4mm,且发酵时间和温度对抑菌活性有影响。
3 strains of antagonistic bacteria were screened from healthy ＇Red Fuji＇ apple peel by the flat panel separation,and the inhibition of antagonistic bacteria to blue mould was studied through the living test and in vitro test.The results showed that the bacteriostatic effect was obvious;the control effect of strain BF-2 reached 87.6%,and the inhibition rate of BF-3 was 33.3% in the confrontation experiment.The antagonistic bacteria were fermented with the beef extract peptone liquid medium,and there was bacteriostatic factor in fermentation medium,and the diameter of bacteriostatic ring caused by strain BF-3 zymotic fluid was 27.4 mm.The antibacterial ability was affected by the fermentation time and temperature.
出处
《北方园艺》
CAS
北大核心
2013年第11期130-132,共3页
Northern Horticulture
关键词
生物防治
细菌
青霉
苹果
采后病害
biological control
bacteria
blue mould
apple
postharvest diseases
