摘要
目的:研究刺五加苷的食用安全性,为促进其合理开发利用提供科学依据。方法:根据《保健食品检验与评价技术规范》,对刺五加苷进行急性毒性实验、小鼠骨髓微核实验、Ames实验、小鼠精子畸变实验、大鼠30d喂养实验。结果:刺五加苷对雌雄小鼠的急性经口的最大耐受剂量(MTD)均大于20g/kg(以体质量计);Ames实验、小鼠骨髓微核实验、小鼠精子畸形实验均为阴性;大鼠30d喂养实验中,实验动物生长情况良好,血液学检查、生化学检查、主要脏体比及组织学检查结果与对照组相比,均无明显差异。结论:刺五加苷属实际无毒物质,未显示有遗传毒性,初步估计其未观察到损害作用的剂量(NOAEL)为20g/kg。
Objective: To evaluate the safety of eleutheroside, and to provide scientific support for the reasonable utilization and development of eleutheroside. Methods: According to the national standard procedures and methods in food safety and toxicological evaluation, a series of toxicological studies on the ingestion of eleutheroside were conducted, which included acute toxicity, genetic toxicity and subacute toxicity tests. Results: For acute toxicity, the maximum tolerated dose (MTD) was higher than 20 g/kg. The results of Ames test, mouse bone marrow micronucleus test and mouse sperm abnormality test were negative. After 30 d of feeding, rats showed no significant difference in body growth, organ development or histological observation. Conclusion: Eleutheroside is actually non-toxic with no genetic toxicity. No adverse effect of eleutheroside at 20 g/kg has been observed during trails for 30 d.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第11期258-262,共5页
Food Science
基金
辽宁省自然科学基金项目(2011205001)
关键词
刺五加苷
急性毒性
遗传毒性
亚急性毒性
食品安全
eteutheroside
acute toxicity
genetic toxicity
subacute toxicity
food safety