摘要
目的探讨朗格罕细胞组织细胞增生症（LCH）病损区朗格罕细胞（LC）超微结构特点，尤其是Birbeck颗粒的超微结构，为Birbeck颗粒的来源、形态、空间结构的研究提供依据。方法收集8例LCH患儿病损部位的病理组织，通过前后固定、脱水、浸透、包埋、聚合等透射电镜常规标本制作过程制作成超薄切片，经染色后在透射电镜下观察病变区LC及Birbeck颗粒的超微结构。结果8例LCH患儿病损区均可见典型LC，其中6例病损区LC胞质中可见成堆或散在分布典型棒状、网球拍样Birbeck颗粒，周围常伴圆形空泡样结构；也可见呈不规则形结构类似Birbeck颗粒的双层膜结构。大部分Birbeck颗粒位于胞质中，部分位于细胞膜内侧，与细胞膜相连续；1例取自多系统病变化疗后复发患儿的病理标本在电镜下观察发现细胞膜外可见类似Birbeck颗粒的棒状双层膜结构，且与细胞膜有形态上联系。结论Birbeck颗粒形态可能具有多样性、不规则性，这些非典型形态的Birbeck颗粒样结构也具有诊断意义。Birbeck颗粒与细胞膜形态学上关系密切，可能起源于细胞膜，空间结构可能为不规则形。
Objective To study the ultrastructural features of Langerhans cell（ LC）, especially Birbeck gran- ules in Langerhans cell histiocytosis （LCH） and provide some uhrastructural evidence to clarify the origin, function and stereoscopic structure of Birbeck granules. Methods Pathologic tissues of 8 LCH patients were treated with routine transmission electron microscope （ TEM ） procedures, fixture, dehydration, permeation, embedding, polymerization, ultra- mierocut, staining and examined by TEM. Results Under the TEM, 8 cases of LCH revealed typical LC cell, and LC cell in 6 cases revealed typical rod, tennnis-racquet like Birbeek granules. Some double membrane structures revealed irregular shape, sometimes accompanied with vacuole-like structure. Most of Birbeck granules locate inside the cytoplas- mic membrane,in continuity with the cell membrane. There was Birbeck granule-like structure outside the cytoplasmic membrane in one case of multisystem LCH. Conclusions The ultrastrueture of Birbeck granules could be various and irregular. The irregular Birbeck granule-like structure can also be useful for the diagnosis of LCH. Birbeck granules may arise from the cell membrane. The stereoscopic structure of Birbeck granules could be irregular.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2013年第9期696-698,共3页
Chinese Journal of Applied Clinical Pediatrics
