摘要
目的:研究卷烟全烟气暴露进行Ames试验的方法,为卷烟烟气的遗传毒性评价提供依据。方法:采用VITROCELLVC10吸烟机、烟气稀释装置和Ames暴露仓,通过调节洁净空气流速、稀释烟气流速、卷烟数等参数,对TA98和TA100进行全烟气暴露的Ames试验。结果:采用5mL/min的稀释烟气和4支卷烟抽吸进行Ames试验时最佳,同时采用30%的S9混合液和无顶层培养基时,卷烟烟气的遗传毒性表现的更为明显。结论:可采用卷烟全烟气暴露的方法进行Ames试验研究,提示其他气体或气溶胶也可采用此方法进行。
OBJECTIVE： To investigate the in vitro exposure and the genetic toxicity of native whole cigarette smoke,and to provide the basis for mutagenic evaluation of cigarette smoke. METHODS：Using VITROCELL VC10 smoke machine,dilution system and Ames exposure module,whole smoke was examined by Ames assay by adjusting the rate of air and diluted smoke,and the number of cigarette. RESULTS：Under the optimum condition, 5 mL/min diluted smoke and 4 cigarettes,whole smoke showed clear genetic toxicity with 30% S9 mixture and without the overlay agar. CONCLUSION：It was possible to analyse the effects of native whole smoke directly and achieve a dose-dependent induction of revertants. The procedure of exposing bacteria directly to gases and complex mixtures offers new possibilities in the field of inhalation genotoxicology for its evaluation in the Ames assay.
出处
《癌变．畸变．突变》
CAS
CSCD
2013年第3期227-231,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
郑州烟草研究院院长科技发展基金项目(332010CA0230)
关键词
全烟气
气溶胶
AMES试验
吸烟机
whole cigarette smoke
aerosol
Ames assay
smoke machine
