摘要
活性污泥菌群结构对于城镇污水处理系统的正常运行至关重要.以《国家城镇污水处理厂出水排放标准GB18918—2002》中出水要求较低的一级B为依据,基于10个已报道的污水处理厂活性污泥样品(8个出水达标,2个不达标)以及4个自行采集的污水处理厂活性污泥样品(3个出水达标,1个不达标)进行了16S rRNA克隆文库对比分析,并在不同分类水平上分别对污水处理厂活性污泥细菌菌群进行了聚类分析.结果表明,在目水平上,出水达标的污水处理厂活性污泥具有相似的细菌菌群结构.另一方面,出水不达标的与正常的污水处理厂虽然在目分类水平上具有相似的物种多样性和均匀度指数,但前者的污泥菌群结构中缺少正常污水处理厂中经常出现的功能细菌黏球菌目(Myxococcales)、黄单胞菌目(Xanthomonadales)、红细菌目(Rhodobacterales)、假单胞菌目(Pseudomonadales)、黄杆菌目(Flavobacteriales)、硝化螺旋菌目(Nitrospirales)和疣微菌目(Verrucomicrobiales)等.本研究方法可为污水处理厂运行状况的快速评估提供科学依据.
To understand characteristics of microbial community structure in activated sludge of wastewater treatment plants （WWTPs）, cluster analysis of microbial community of ten as-reported and other four newly taken activated sludge samples was made based on 16S rRNA clone library database. Similar microbial community structures were found for function normal WWTPs at order level. However, function failed WWTPs with poor effluent quality unable to meet the B standard level 1 required by the urban sewage treatment plant emission standards （GB18918--2002） had less shared bacteria than the normal, although they had similar biodiversity and evenness in the microbial community. At order level, function failed WWTPs lack of shared bacteria often existed in function normal WWTPs, which included Myxococcales, Xanthomonadale, Rhodobacterales, Pseudomonadales, Flavobacteriales, Verrucomicrobiales and Nitrospirales. These bacteria played very important role in the removal of organics and nitrogen from wastewater. The present study was of great help to efficient evaluation of the health status of WWTPs.
出处
《应用基础与工程科学学报》
EI
CSCD
北大核心
2013年第3期522-531,共10页
Journal of Basic Science and Engineering
基金
广州市水务局水务科技项目(GZSK-FW-1201)
关键词
克隆文库
聚类分析
目
污水处理厂
microbial community structure
clone library
cluster analysis
order
WWTPs
