摘要
目的探讨黄芩苷对人喉癌Hep-2细胞血管生成调节因子[基质金属蛋白酶（matrix metalloproteinase, MMP ）、血管内皮生长因子（ vascular endothelial growth factor, VEGF）、碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）]表达及迁移能力的影响。方法体外培养Hep-2细胞，（1）加人佛波脂诱导，明胶酶谱法和Transwell小室分别观察不同浓度黄芩苷对Hep-2细胞MMP表达水平及迁移能力的影响；（2）不加佛波脂，酶联免疫吸附测定（ELISA）法观察不同浓度黄芩苷对细胞VEGF和bFGF蛋白含量的影响。结果黄芩苷低、中、高剂量组MMP-3酶活性[（95．0±4．9）％、（93．1±6．2）％、（90．7±7．3）％vs（100．0±0．0）％，t=2．123，2．309，2．412，P〈0．051、MMP-9酶活性[（92．3±7．5）％、（89．5±9．3）％、（85．6±6．1）％vs（100．0±0．0）％，t=2．253，2．302，3．708，P〈0．05或P〈0．01]、VEGF蛋白含量[（230．6±12．7）ng/L、（212．1±15．9）ng／L、（184．2±23．5）ng／Lvs（2d2．7±9．5）ng／L，t=2．408，3．733，4．146，P〈0．05或P〈0．01]、bFGF蛋白含量[（181．2±13．0）ne／L、（169．9±17．3）ng／L、（140．7±9．2）ng／Lvs（190．7±8．2）ng／L，t=2．330，2．922，5．145，P〈0．05或P〈0．01]及细胞迁移率[（32．9±7．1）％、（27．9±4．4）％、（23．3±6．0）％vs（38．3±5．6）％，t=2．141，3．365，4．027，P〈0．05或P〈0．01]与对照组比较，差异均有统计学意义。结论通过抑制血管生成调节因子的表达，黄芩苷可有效抑制人喉癌Hep-2细胞的迁移能力，可能用于喉癌的治疗。
Objective To explore the effects of bacailin on the angiogenic regulating factor and mi- gration activity of laryngeal cancer cells. Methods Hep-2 was cultured in vitro, （1）in the groups adding phorbol ester, gelatin-zymogram and Transwell assay were used to observe the effects of bacailin on the gela- tinase activity of matrix metalloproteinase （MMP） and the migration ability of Hep-2, respectively; （2）in the group without phorbol ester, enzyme-linked immunosorbent assay （ELISA） was used to detect the effects of bacailin on the protein level of endothelial growth factor （VEGF） and basic fibroblast growth factor （ bF- GF）. Results Among the control group, the low-dose group, the middle-dose group, and the high-dose group, the activity of MMP-3 of each corresponding group was （ 100. 0 ± 0. 0） %, （95.0 ± 4. 9） %, （93.1 ±6. 2）%, and （90. 7 ±7.3 ）% with statistically significant difference （ t = 2. 123,2. 309,2. 412, P 〈 0. 05 ） ; the activity of MMP-9 of each corresponding group was （ 100. 0 ± 0.0 ） %, （ 92. 3 ± 7.5 ） % , （89.5 ±9.3）% , and （85.6 ±6.1）% with statistically significant difference （ t =2. 253,2. 302,3. 708, P 〈 0. 05 or P 〈 0. 01 ） ; the protein level of VEGF of each corresponding group was （ 242.7 ± 9.5 ） ng/L, （230. 6 ± 12.7）rig/L, （212. 1 ± 15.9）rig/L, and （ 184.2 ±23.5）ng/L with statistically significant differ- ence （ t =2. 408,3. 733,4. 146, P 〈0.05 or P 〈0.01 ） ; the protein level of bFGF of each corresponding group was （190.7 ±8.2） ng/L, （181.2 ±13.0） ng/L, （169.9 ±17.3）ng/L, and （140.7 ±9.2）ng/Lwith statistically significant difference （ t = 2. 330,2. 922,5. 145, P 〈 0. 05 or P 〈 0. 01 ） ; Cellular migra- tion rate of each corresponding group was （38.3 ±5.6）%, （32.9 ±7.1）% , （27.9±4.4）% , and （23.3 ± 6. 0）% with statistically significant difference （ t = 2. 141,3. 365,4. 027, P 〈 0.05 or P 〈 0. 01 ）. Conclusions Bacailin could suppress the migration of Hep-2 through inhibiting expression of an- giogenic regulating factor, which might has clinical value in the treatment of laryngeal cancer.
出处
《中国医师杂志》
CAS
2013年第5期640-642,共3页
Journal of Chinese Physician
