摘要
根据编码人IgG1Fc(Fcγ1 )的基因序列 ,设计合成 1对与之相匹配的引物。采用RT PCR法 ,从正常人白细胞mRNA中扩增获得预期目的基因Fcγ1 70 0bp基因片段 ,成功构建PUC1 8 Fcγ1 70 0 重组克隆载体 ,酶切、酶谱分析与预期结果相符 ;DNA测序结果与GenBank报道一致。
According to the cDNA sequences which encode the human IgG1Fc, a pair of primers were designed; The expected gene (Fcγ1 700 bp ) which encode Fcγ1 was amplified by RT PCR from mRNA extracted from normal person′s leukocyte; PUC18 Fcγ1 700 recombinant cloning vector was successfully constructed, and the enzyme digestion analysis are identical to expected results. Sequences are identical to those in GenBank report.
出处
《首都医科大学学报》
CAS
2000年第2期93-96,共4页
Journal of Capital Medical University
基金
国家自然科学基金资助项目 !(395 70 6 6 6 )
