摘要
ＴＦＰ（１０－１００μｍｏｌ／Ｌ）可引起裂殖酵母（Ｓｃｈｉｚｏｓａｃｃｈａｒｏｍｙｃｅｓ ｐｏｍｂｅ）胞外Ｃａ２＋内流，ＴＦＰ浓度不同，促进Ｃａ２＋内流程度也不一样，５０μｍｏｌ／ＬＴＦＰ的促进作用最大。并且ＴＦＰ浓度越大，Ｃａ２＋内流出现峰值也越早，１０、２０、５０、１００μｍｏｌ／ＬＴＦＰ处理后，胞内总钙出现峰值时间分别为４５、４５、３０、１５分钟。胞外Ｈ＋浓度也会对ＴＦＰ引起的Ｃａ２＋内流产生不同影响，缓冲液的ｐＨ值为６．０时最有利于ＴＦＰ引起胞内Ｃａ２＋含量增加，碱性条件下ＴＦＰ的效果最不明显。由ＴＦＰ引起的Ｃａ２＋内流增加要比单一地增加外钙浓度效果好得多，ＴＦＰ在１０μｍｏｌ／Ｌ浓度的外钙条件下引起的胞内钙含量数值比１０００μｍｏｌ／Ｌ的外钙条件而无ＴＦＰＴ所引起的胞内钙含量还要高５３．９％。缓冲液中加入０．８％的钙离子通道阻断剂ＬａＣ１３或溶液中无葡萄糖的存在，ＴＦＰ的促进作用消失，说明ＴＦＰ促进Ｃａ２＋内流是通过钙离子通道来完成的并需要能量参与。
Trifluoperazine(10-100mol/L) induced Ca2+ influx into Schizosaccharomyces pombe cells andthe effect of stimulation was doze-depedent,in which 50μmol / L TFP had the most appent effecL With theconcentration of TFP increasing, the peak of Ca2 + influx would appeared earlier. By adding 10, 20. 50. 100umol /L TFP respechvely, the peak appeared at the bine of 45.45, 30. 15adn later. The effect of shmulahon inducedby TFP was better than addition exogenous Ca2+ alone, suggesting that TFP and exogenous Ca2+ had thecooperahve effect. The stimulation induced by TFP was observed only in the presence of metabolic substrate andwas completely iambited by 0.8% LaCI3 The results indicated that the shmulation of Ca2+ influx wasenergy-depedent carrier-mediated process.
出处
《菌物系统》
CAS
CSCD
北大核心
2000年第1期97-101,共5页
Mycosystema
基金
江苏省自然科学基金
